【作者】 黄芳； 黄晓兰； 吴惠勤； 邓欣； 罗辉泰； 朱志鑫； 林晓珊；

【Author】 HUANG Fang,HUANG Xiao-lan,WU Hui-qin,DENG Xin,LUO Hui-tai, ZHU Zhi-xin,LIN Xiao-shan (Guangdong Provincial Public Laboratory of Analysis and Testing Technology,China National Analytical Center(Guangzhou),Guangzhou 510070,China)

【机构】 中国广州分析测试中心广东省分析测试技术公共实验室；

【摘要】 建立了枸杞子药材中甜菜碱的高效液相色谱-质谱测定方法。优化了样品提取方法、液相色谱条件和质谱参数。样品用60%甲醇超声提取,高效液相色谱分离,色谱柱为正相硅胶柱,流动相为乙腈-水(均含有0.2%的甲酸)(体积比75:25),流速0.4 mL/min。采用正离子模式的电喷雾质谱检测,多反应选择离子检测(MRM),检测离子对为:m/z 118→58;m/z 118→59。方法简便快速、准确可靠,已用于实际样品的测定。更多还原

【Abstract】 A method was proposed for the determination of betaine in fruits of Lycium barbarum L. by high-performance liquid chromatograph - mass spectrometry(HPLC - MS).The conditions of extraction procedures and parameters of HPLC - MS were optimized.Betaine was extracted with methanol - water(60:40,by volume),then separated on an Agilent ZORBAX RX - SIL column.Mixture of acetonitrile and water(75:25,by volume)(additional 0.1%formic acid added) was used as mobile phase,and detected with positive-ion electrospray ionization mass spectrometry(ESI MS) under multiple reaction monitoring(MRM) mode.The results indicated that the method was rapid, selective,sensitive and stable enough to examine betaine in fruits of Lycium barbarum L.更多还原