【作者】 石磊； 程舸； 于雷； 刘晓梅； 王韶；

【Author】 SHI Lei,CHENG Ge,YU Lei,LIU Xiao-mei,WANG Shao (College of Public Health,Jilin University,Changchun 130021,China)

【机构】 吉林大学公共卫生学院；

【摘要】 <正>傅立叶变换离子回旋共振质谱(Fourier transform ion cyclotron resonance mass spectrometry,FTICR-MS),具有经典质谱及现代质谱技术的多种功能,由于采用离子回旋共振质量检测器,FT-ICRMS具有超高分辨率、超高灵敏度(fmol),以及较宽的质量范围(m/z>10 000 u)。多级质谱功能与肽库配合越来越广泛地应用到生物大分子的研究中,已经成为生命科学相关前沿领域如蛋白质组研究不可缺少的工具之一。更多还原

【Abstract】 FT-ICR-MS technology has significant sensitivity and dynamic range,enabling the detection of different fragmentations of peptides and proteins at lower and lower levels.In the bottom-up approach,by far the most frequently used proteomics approach,a single LC-MS run can reveal tens and even hundreds of useful information.For each peptide selected for MS/MS,two data-files were generated,one for ECD and another one for CAD.They were merged as described and submitted to the Mascot search engine using the protein database. These thresholds ensure almost 100%statistical confident in the peptide identification.更多还原