【作者】 王艳玲； 向成钢； 王长林； 张雪梅； 王迎杰； 尹玲；

【Author】 Wang Yan-ling 1,Xiang Cheng-gang 1,Zhang Xue-mei 2,Wang Chang-lin 1,Wang Ying-jie 1, Yin ling 1 (1.Institute of Vegetables & Flowers,Chinese A cademy of Agricultural Sciences,Beijing 100081;2.Beijing Agricultural Technology Extension Station,Beijing 100029)

【机构】 中国农业科学院蔬菜花卉研究所； 北京市农业技术推广站；

【摘要】 将21份南瓜属供试材料(美洲南瓜、中国南瓜、印度南瓜)按照不同类群进行分类,以390对SSR引物对21份材料进行多态性分析。结果表明:供试材料之间具有丰富的遗传多样性,美洲南瓜与中国南瓜亲缘关系较近,二者与印度南瓜的亲缘关系较远;同一亚种内及同一栽培类群内材料之间具有更近的亲缘关系;SSR在不同材料中的多态性潜力受其所包含的重复单元数目及其碱基组成的影响,重复单元中的碱基数量越多、重复单元数目越多则多态性潜力越大。更多还原

【Abstract】 Twenty-one accessions of Cucurbita genus(C.pepo、C.moschata、C.maxima) were classified into different cultival groups,then studied their genetic relationships,using 390 SSR markers. The SSR sequences and sources were discussed also.The results showed that the level of polymorphism within cultivals was very high,there are general similarity between the cultival-groups methods and molecular methods.The relation between C.pepo and C.moschata was closer than that between C.moschata and C.maxima by clustering.The potential of SSR polymorphism were affected by the motifs number and consistence of nucleotides of SSR.Different distance measures could have different results in the evolutionary relationship studing.更多还原