【作者】 童庆宣； 刘育梅； 胡宏友； 宋志瑜； 李学梅； 卢昌义；

【Author】 TONG Qing-xuan~1 LIU Yu-mei~1 HU Hong-you~2 SONG Zhi-Yu~3 LI Xue-mei~2 LU Chang-yi~2 (1 Xiamen overseas Chinese subtropical plant introduction garden,Xiamen 361002;2 College of Oceanography and Environmental Science of Xiamen University,Xiamen 361005;3 Fujian Institute of Subtropical Botany,Xiamen 361006)

【机构】 厦门华侨亚热带植物引种园； 厦门大学海洋与环境学院； 福建省亚热带植物研究所；

【摘要】 为研究人心果[Manilkarazapota(L.)van Royen]的耐盐能力及其在NaCl胁迫下渗透调节物质、抗氧化酶活性的变化,探讨人心果在NaCl胁迫下的适应机制并为果树的耐盐机理提供理论依据,采用盆栽试验法,分别用0、2‰、4‰、6‰、8‰NaCl溶液对人心果进行了胁迫处理,测定了人心果叶片的叶绿素、游离脯氨酸、可溶性蛋白、可溶性糖、丙二醛的含量和SOD、CAT、POD的活性以及盆土的实际盐度。结果表明:在NaCl胁迫下,人心果叶片的叶绿素合成受到明显抑制;SOD活性随处理浓度增加而升高,且在各处理浓度下活性均显著高于对照,POD和CAT活性随浓度增加呈现先升后降趋势,POD活性在2‰处理浓度时达到最高,CAT活性在4‰处理浓度时达到最高;脯氨酸、可溶性蛋白含量与处理浓度呈现正相关,可溶性糖、丙二醛的含量与处理浓度呈现负相关,4者含量在各处理浓度下均和对照有显著差异(除可溶性蛋白在2‰处理浓度外),推测有机小分子作为渗透调节物质在人心果受NaCl胁迫过程中起着重要的作用。更多还原

【Abstract】 In this paper,the eco-physiological characteristics of NaCl stress in Manilkara zapota(L ) van Royen leaves had been researched by watering the potted plants with 0,2‰,4‰,6‰,8‰NaCl.The characteristics included the changes about chlorophyll,proline,soluble protein,soluble sugar,SOD,POD,CAT,MDA.Through the eco-physiological response to NaCl stress,the NaCl tolerance of M.zapota had been learned and the theory about salt tolerance mechanism had been enriched also.The results showed that the chlorophyll synthesis were significantly restricted under the NaCl.The activity of the SOD increased under the NaCl stress,however the change of the activities of POD or CAT was not the same.The activity of POD or CAT increased first and then droped.The POD activity reached a high under 2‰NaCl,so did CAT activity under 4‰NaCl.stress.The content of proline or soluble protein increased while that of soluble sugar or MDA droped under NaCl stress. The contents of the 4 substances under NaCl stress differed significantly from that in the control except for soluble protein under 2‰NaCl stress.The small organic molecules working as osmotic potentials in cells played a key role in salt tolerance of M. zapota.更多还原