【作者】 孙霞； 郑成淑； 王秀峰； 邢世岩； 束怀瑞；

【Author】 SUN Xia~(1,3) ZHENG Cheng-shu~(1,3) WANG Xiu-feng~(1,3) XING Shi-yan~(2,3) SHU Huai-rui~(1,3,4) (1 College of Horticulture Science and Engineering,Shandong Agricultural University;2 College of Forestry,Shandong Agricultural University;3 State Key Laboratory of Crop Biology; 4 National Engineering and Technique Center of Apples,Tai’an 271018)

【机构】 山东农业大学园艺科学与工程学院； 作物生物学国家重点实验室； 山东农业大学林学院； 国家苹果工程技术中心；

【摘要】 研究菊花花芽分化期转录组表达序列标签(ESTs)。以切花菊品种‘神马’为试材,从菊花不同花芽分化期叶片和茎尖中分别提取的总RNA构建cDNA文库,利用高通量EST测序技术,分析菊花花芽分化期叶片和茎尖的转录组表达序列标签(ESTs)。cDNA克隆测序共获有957 720条片段重叠群(contigs)。组装后筛选出113 515条非冗余重叠群(unigenes)。对所有unigenes进行GO功能分类,共收集到54 488条分属于分子功能、细胞组分和生物学过程的unigenes,占总unigenes的48.0%。其中,参与分子功能的共12种,涉及13837条unigenes(12.2%);参与细胞组分的共11种,涉及24 353条unigenes(21.5%);参与生物过程的共18种,涉及16 298条unigenes(14.4%);用BLAST比对所有unigenes序列,得到编码蛋白框(CDS)的核酸序列和氨基酸序列均为54 644条,其中53 110条(97.2%)核酸序列是完整无间隔(不含N)的序列。用ESTscan预测得到其编码区的核酸序列和氨基酸序列均为8 506条,其中7 871条(92.5%)的核酸序列是完整无间隙(不含N)的序列。将所有unigenes与COG数据库BLAST比对显示,共有22 871条(19.5%)与已知其他植物同源,并被归为23类直系同源簇群,未知功能的有780条(3.5%),推测可能是菊花新基因;根据KEGG注释信息的预测结果,共得到25 001条参与代谢通路的unigenes,从中获得植物节律代谢通路相关unigenes共269条(1.08%),对其进行BLAST比对,获得参与光周期途径的光受体基因、生物节律钟基因和生物节律钟输入和输出基因等相似功能基因共20个。获得了菊花花芽分化期叶片和茎尖中转录组的ESTs,将所获得unigenes提交到公共数据库中,丰富菊花花芽分化期的ESTs信息,为菊花开花相关基因的筛选和后期转基因利用奠定了基础。更多还原

【Abstract】 Studies on transcriptomic express sequence tags(ESTs) of the leaves and apical tips of chrysanthemum during floral differentiation.Analysis of the transcriptomic express sequence tags(ESTs) of the leaves and apical tips of chrysanthemum during floral differentiation with chrysanthemum varieties‘Jinba’ as the experiment materials by high-throughput EST sequencing technique,and by construction of cDNA liberary from extracted total RNA of the leaves and apical tips of chrysanthemum during floral differentiation.cDNA clones were sequenced,and 957 720 contigs were sellected,and then the contigs were further assembled into 113 515 unigenes.All of those unigenes were function classified by GO software,and sellected 54 488 unigenes which of moleculor functions,cell conpenents and bioprocesses involved 48%of all unigenes.All of the above unigenes invoved 12 of molecular functions with 13 837 unigenes(12.2%),and of cell components of with 24 353 unigenes(21.5%), and of bioprocesses with 16 298 unigenes(14.4%).Sequences of nucleic acids and amino acids of 54 644 encoding protein boxes(CDS) were sellected from all of the unigenes of chrysandiemum during floral differentiation by BLAST software.Among them there were 53 110(97.2%) unigene sequences of nucleic acids complete without break(excluding N) sequence.Also 8 506 unigene sequences of nucleic acids and amino acids of encoding protein boxes(CDS) were cellected from all of the unigenes of chrysanthemum during floral differentiation by ESTscan software.Among them there were 7 871(92.5%) unigene sequences of nucleic acids complete without break(excluding N) sequence.Comparison to the GenBank non-redundant(nr) database in COG database revealed that 22 871 unigenes(19.5%) sequences were similar to other known plant homologous genes which classfied to 23 homologys and 780 unigenes sequences(3.5%) maybe new genes in chrysanthemum.Also 25 001 unigenes were cellected which of participating in metabolism pathway using KEGG database.From there 269 unigenes (1.08%) also cellected that related to participating circadian rhythm metabolism pathways,and also cellected 20 homologous genes which related to photoperiod pathways including photoreceptor genes,circadian rhythm clock genes,circadian rhythm import and export genes.Transcriptome ESTs of the leaves and apical tips of chrysanthemum during floral differentiation were cellected. Those unigenes presented here will be submitted to the public database,and they will be valuable additions to floral differentiation expression sequence tags ESTs data,so the information might be the base of sellecting flowering related genes and using for transgenes.更多还原