节点文献
我国石斛属部分植物的ISSR标记分析
Analysis of Relationships among the Part Species of Dendrobium Based on Inter-Simple Sequence Repeat(ISSR)
【Author】 WU Rong-hua~(1,2) WANG Yan~1 PENG Zhen-hua~1 LI Zhen-jian~1 (1 Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration Research Institute of Forestry,CAF, Beijing 100091;2 Forestry and Horticultural College of Henan Agricultural University,Zhengzhou 450002)
【机构】 中国林业科学研究院林业研究所国家林业局林木培育重点实验室； 河南农业大学林学园艺学院；
【摘要】 用ISSR技术研究了石斛属32种植物原生种之间的亲缘关系。从50条引物中筛选出5条引物用于ISSR扩增,共得到103条清晰稳定的谱带,其中多态性谱带93条,多态比率90.2%。利用UPGMA聚类分析,将32种石斛划分为6个类群。根据聚类分析,讨论了石斛属32种植物的种间亲缘关系。
【Abstract】 The technique of inter-simple sequence repeat(ISSR) was used to study the relationships of 32 species of Dendrobium. 5 primers were selected from 50 ISSR primers.103 DNA fragments were amplified from 32 samples and each primer resulted in DNA fragments.Fragments were polymorphic(percentage of polymorphic bands was 90.2%).Cluster analysis with UPGMA method showed that 32 species of Dendrobium tested in this research could be divided into six groups.By analysis of molecular dendrogram,the genetic relationships among 32 species of Dendrobium were discussed.
- 【会议录名称】 中国观赏园艺研究进展（2010）
- 【会议名称】观赏园艺与西部发展——中国园艺学会观赏园艺专业委员会2010年全国学术年会
- 【会议时间】2010-07-01
- 【会议地点】中国青海西宁
- 【分类号】S567.239
- 【主办单位】中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心