【作者】 律春燕； 王雁； 朱向涛；

【Author】 Lü Chun-yan WANG Yan ZHU Xiang-tao (Research Institute of Forestry,CAF,SFA Key of Tree Breeding and Cultivation,Beijing 100091)

【机构】 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室；

【摘要】 以黄牡丹的新鲜花粉为试材,利用单因子试验比较了液体培养基中蔗糖浓度、硼、钙、镁、钾及pH值对黄牡丹花粉萌发的影响,在此基础上进行了正交试验,比较了蔗糖、H₃BO₃及CaCl₂对黄牡丹花粉萌发的影响;通过对醋酸洋红染色法、I-KI染色法和TTC染色法的比较,寻找快速测定黄牡丹花粉生活力的方法。试验结果表明:蔗糖及H₃BO₃对黄牡丹花粉萌发有极显著影响。在pH值为6.0时,蔗糖150g·L^-1+H₃BO₃30mg·L^-1+CaCl₂40mg·L^-1适宜黄牡丹花粉培养,纯水培养没有造成花粉原生质体破裂,内含物外流,但萌发率极低;高浓度的蔗糖和盐会造成原生质体失水萎缩,质壁分离,这两种情况都抑制花粉萌发。TTC染色法是快速测定黄牡丹花粉生活力的最适染色法。更多还原

【Abstract】 The fresh pollen of Paeonia lutea was employed as experimental material to carry on the studies on culture solution for the determination of pollen viability.Comparisons were made among the effects of different concentration of sucrose,boron,calcium,magnesium, potassium and pH values on the germination of Paeonia lutea’s pollen through simple factorial experiment.And an orthogonal design was used on that basis to compare the effects of sucrose,boron and calcium on the germination of Paeonia lutea’s pollen.Carmine acetate staining、I₂-KI staining、TTC staining were compared in order to seek for a fast determination method of Paeonia lutea’s pollen viability.Results showed that sucrose and boron have great effects on the germination of Paeonia lutea’s pollen.Under the optimum pH6.0 values,the optimum culture solution was sucrose150g·L^-1+H₃BO₃30mg·L^-1+CaCl₂40mg·L^-1.When cultured in pure water,the protoplasm of the pollen wouldn’t split,and the substance inside wouldn’t flowout,but its germination rate is very low; when cultured in the solution with high concentration of sucrose or salt,the protoplasm would dehydrate and separate from the cell wall.These two would inhibit the germination of Paeonia lutea’s pollen.And TTC staining was the optimum staining for the fast determination of Paeonia lutea’s pollen viability.更多还原