CREB磷酸化改变促进莲房原花青素改善老年认知障碍大鼠学习记忆能力的机制研究

【作者】 许继取； 荣爽； 张丽； 杨雪锋； 郝丽萍； 姚平； 刘烈刚；

【机构】 华中科技大学同济医学院营养与食品卫生学系；

【摘要】 本研究主要的目的是确定莲房原花青素(LSPC)对老年认知障碍大鼠海马和皮质CREB磷酸化状态的调节作用。根据Morris水迷宫的结果从老年大鼠中筛选出老年认知正常(AU)和老年认知障碍(AI)大鼠。与年轻组和AU组相比,AI组海马CREB磷酸化程度以及BDNFmRNA和蛋白表达明显降低。另外,AI大鼠海马ERK 42/44以及CaMKIV的磷酸化状态也明显降低。LSPC(50,100 mg/kg BW)灌胃7周明显逆转除CaMKIV磷酸化状态以外所有指标的降低。表明LSPC可活化ERK通路而增强海马CREB依赖性转录,从而改善AI大鼠的学习记忆能力。更多还原

【Abstract】 The major purpose of this study was to determine the effects of procyanidins extracted from the lotus seedpod(LSPC) on cAMP-response element-binding protein(CREB) phosphorylation in hippocampus and cerebral cortex in cognitively impaired aged rats.Based on Morris water maze,aged-unimpaired(AU) and aged-impaired(AI) rats were chosen from aged rats.Comparing with young and AU animals,AI rats exhibited significant reduction in hippocampal CREB phosphorylation states as well as BDNF mRNA and protein expressions, which were accompanied by decreased phosphorylation states of hippocampal ERK 42/44 and CaMKIV.LSPC supplementation(50,100 mg/kg BW,p.o.) for 7 weeks significantly reversed all these declines happened in hippocampus except CaMKIV phosphorylation levels. These results suggested LSPC might enhance CREB-dependent transcription through the activation of ERK signalling pathway,which might contribute to its ameliorative effects on cognitive deficits in AI animals.更多还原