【作者】 陈恒雷； 万红贵； 吕长武； 曾宪贤；

【Author】 CHEN Heng-lei~(1,2),WAN Hong-gui~1,LV Chang-wu~2,ZENG Xian-xian~(2,) (1.College of Life Science and Pharmacy Engineering,Nanjing University of Technology,Nanjing 210009,China;2.Ion beam bio-engineering center,Xin Jiang University,Urumchi 830008,China)

【机构】 南京工业大学制药与生命科学学院； 新疆大学物理学院离子束生物工程中心；

【摘要】 通过能量15 keV、剂量1.2×10¹⁶cm^-2的低能N⁺束梯次注入、反复筛选,应用营养缺陷型筛选办法初筛、摇瓶发酵法复筛获得了2株菌丝体多糖产量较出发菌株分别提高了46.55%、75.14%且遗传稳定的阿魏菇多糖高产菌PFPH-1、PFPH-2。采用深层发酵法培养阿魏菇菌丝体,通过添加不同的碳、氮源、无机盐和改变pH检测菌丝体干重及胞内多糖的积累,发现阿魏菇深层发酵体系中有利于菌丝体生物量积累及胞内多糖合成的最佳组合为1.0%小麦粉、2.0%蔗糖、2.0%的黄豆粉、1.5%麸皮（煮汁）、0.2%K₂HPO₄和0.15%MgSO₃·7HO₂,最适pH为5.64。正交优化组合对阿魏菇多糖高产菌PFPH-2菌丝体胞内多糖积累有协同效应,组合后的发酵体系中菌丝体胞内多糖产量达到903.73±1.23 mg·L^-1的最高水平。更多还原

【Abstract】 Pleurotus polysaccharide high-yield strains were selected through a method of auxotrophic primary screening and Shake-flask fermentation re-screening after low-energy heavy ions(the fluence of 1.2×10¹⁶N⁺/cm² at the energy of 15 keV) stepwise implantation.Two Pleurotus polysaccharide high-yield strains,PFPH-1 and PFPH-2,were selected with stable mycelium polysaccharide yield.The mycelium polysaccharide yield of PFPH-1 and PFPH-2 increased by 46.55%and 75.14%,respectively,compared to the original strain.The accumulation of mycelium biomass and intracellular polysaccharides were monitored in the submerged fermentation of Pleurotus ferulae by supplementation of various carbon and nitrogen sources as well as inorganic salts and pH alteration.The optimal submerged fermentation medium favoring the accumulation of mycelium biomass and intracellular polysaccharides of PFPH-2 consisted of 1.0%wheat flour,2.0%sucrose, 2.0%soybean flour,1.5%bran extract,0.2%K₂HPO₄,and 0.15%MgSO₄·7H₂O,with a fittest pH value of 5.64.The orthogonal combination of the optimal carbon and nitrogen sources with inorganic salts indicates a synergistic effect on the accumulation of mycelium biomass and intracellular polysaccharides in the submerged fermentation of PFPH-2.The yield of mycelium polysaccharides of PFPH-2 increased to 903.73±1.23 mg·L^-1 by the end of fermentation.更多还原