【作者】 焦本蒸； 马淑梅； 易贺庆； 孔德娟； 赵广通； 高琳； 刘晓冬；

【Author】 JIAO Ben-zheng~1,MA Shu-mei~1,YI Heqing~1,KONG De-juan~1, ZHAO Guang-tong~1,GAO Lin~1,LIU Xiao-dong~(1,2*) (1.Key Lab of Radiobiology(Ministry of Health),Jilin University,Changchun 130021,China;2.China-Japan Union Hospital,Jilin University,Changchun 130000,China)

【机构】 吉林大学公共卫生学院卫生部放射生物学重点实验室； 吉林大学中日联谊医院放射肿瘤科；

【摘要】 目的探讨表观遗传学调控与辐射尤其是低剂量辐射的关系。方法采用文献回顾及综合分析的手段进行研究。结果DNA甲基化与辐射尤其是低剂量辐射互为调控;组蛋白甲基化对辐射的调控主要存在于高剂量辐射领域,组蛋白乙酰化酶抑制剂在增强辐射敏感性方面有着广泛的应用前景;虽然γ-H2AX在1-15Gy照射范围未见与辐射敏感性相关,组蛋白泛素化在DNA损伤修复机制中发挥重要作用。染色体重塑在DNA双链断裂修复和染色体反应中发挥综合作用。辐射对microRNA表达的影响因细胞种类、辐射剂量、照射时间等而异。结论有关表观遗传学调控与辐射尤其是低剂量辐射的关系方面的研究尚存在诸多盲点,在低剂量辐射反应中很多新的调控元素将会被证实,为机制研究、辐射防护、肿瘤治疗方面提供新的思路。更多还原

【Abstract】 Purpose:To conclude the relationship between epigenetics regulation and radiation responses,especially in low-dose area.Methods:The literature was examined for papers related to the topics of DNA methylation,histone modifications,chromatin remodeling and non-coding RNA modulation in low-dose radiation responses.Results:DNA methylation and radiation can regulate reciprocally,especially in low-dose radiation responses.The relationship between histone methylation and radiation mainly exists in the high-dose radiation area;histone deacetylase(HDAC) inhibitors show a promising application to enhance radiation sensitivity,no matter whether in low-dose or high-dose areas;the connection betweenγ-H2AX and LDR has been remained unknown,althoughγ-H2AX has been shown no radiation sensitivities with 1-15Gy irradiation;histone ubiquitination play an important role in DNA damage repair mechanism.Moreover,chromatin remodeling has an integral role in DSB repair and the chromatin response,in general,may be precede DNA end resection. Finally,the effect of radiation on miRNA expression seems to vary according to cell type,radiation dose,and post-irradiation time point.Conclusion:Although the advance of epigenetic regulation on radiation responses,which we are managing to elucidate in this review,has been concluded,there are many questions and blind blots deserved to investigated,especially in low-dose radiation area.However,as progress on epigenetics,we believe that many new elements will be identified in the low-dose radiation responses which may put new sights into the mechanisms of radiation responses and radiotherapy.更多还原