【作者】 王萌； 任国峰；

【Author】 WANG Meng,REN Guofeng Department of Nutrition and Food Hygiene,School of Public Health,Central South University,Changsha 410078,China

【机构】 中南大学公共卫生学院营养与食品卫生学系；

【摘要】 目的研究雌马酚对人乳腺癌细胞MCF-7增殖的抑制作用并初步探讨其分子机制。方法利用MTT法测定不同浓度(0.1、0.5、1、5和10μmol/L)雌马酚对MCF-7细胞增殖率的影响,流式细胞仪测定雌马酚对细胞凋亡率和细胞周期的影响,Westernblot测定bag-1、bcl-2、VEGF、ERK1/2、p-ERK1/2、p38以及p-p38蛋白表达。结果随着雌马酚浓度升高,人乳腺癌细胞MCF-7的增殖率下降(P<0.05),细胞凋亡率升高,细胞周期被阻滞于G0/G1期,雌马酚可以下调bag-1、bcl-2、VEGF、p-ERK1/2以及p-p38蛋白表达,并具有一定剂量-效应关系。结论雌马酚可以通过促进细胞凋亡并阻滞细胞周期于G0/G1期,以及下调bag-1、bcl-2、VEGF蛋白、抑制ERK1/2和p38蛋白磷酸化的方式实现对人乳腺癌细胞MCF-7增殖的抑制。更多还原

【Abstract】 Objective To investigate the proliferation inhibition effects of equol on human breast cancer cell line MCF-7 and explore its molecular mechanisms. Methods MCF-7 cells were treated with different concentration of equol,including 0. 1,0. 5,1,5 and 10 μmol / L. After the treatment,the proliferation rate of cells was examined by MTT method and the cell apoptosis percentage and cell cycle phase were determined by flow cytometry. The bag-1,bcl-2,VEGF,ERK1 /2,p-ERK1 /2,p38 and p-p38 protein were determined using Western blotting. Results A dose and time effect of proliferation inhibition of equol was proved in MCF-7. The MCF-7 cell apoptosis percentage increased significantly in the groups of equol,and the cell cycle arrest in G 0 / G 1 phase. The expression of bag-1, bcl-2, VEGF, p-ERK1 /2 and p-p38 protein were decreased gradually with the increase concentration of equol. Conclusion The equol could inhibit the proliferation of the breast cancer cell lines MCF-7 and its inhibitory effect may be due to inducing apoptosis,arresting the cell cycle in G 0 / G 1 phase,down-regulating the expression of bag-1,bcl-2,VEGF,p-ERK1 /2 and p-p38 protein.更多还原