枸杞多糖对糖尿病肾病兔氧化应激反应的影响

【作者】 耿天琪； 罗琼； 闫俊； 李菁菁； 赵颀涵；

【机构】 武汉大学公共卫生学院营养与食品卫生学系；

【摘要】 目的:研究构杞多糖对糖尿病肾病兔氧化应激反应的影响。方法:采用四氧嘧啶(ALX)诱导糖尿病肾病兔动物模型。将20只雄性日本大耳白兔随机分为5组,每组4只,分别为正常对照组、阴性对照组、预防组、治疗组和阳性对照组。预防组于糖尿病(DM)成模后即灌胃LBP(10mg/kg)持续12w,治疗组和阳性组于糖尿病肾病(DN)成模(第8w)后分别灌胃LBP(10mg/kg)和美卡素(3.7mg/kg)持续4w。在造模后12周处死动物。日常监测动物血糖,尿蛋白,动物处死后取肾脏检测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性;一氧化氮(NO)、一氧化氮合成酶(NOS)含量,观察枸杞多糖对糖尿病肾病兔氧化应激反应的影响。结果:预防组与阴性组相比血糖水平明显较低、尿蛋白水平低(P<0.05),SOD含量较阴性组多,MDA较阴性组少(P<0.05),NO和NOS水平也优于其他组(P<0.05)。结论:枸杞多糖对糖尿病肾病兔的抗氧化应激作用可能是其发挥治疗作用的机制之一。更多还原

【Abstract】 Objective:To investigate the effect of Lycium barbarum polysaccharides(LBP) on oxidative stress reaction in renal function of diabetic nephrosisi rabbits.Methods:Diabetes was induced by injecting alloxan(ALX) .Japanese white rabbits were randomly divided into 5 groups:normal control group,negative control group,prevention group,treatment group,positive control group(n = 4 for each group).LBP(lOmg/kg) was given to prevention group after DM model successful for 12 weeks and treatment group after DN model successful for 4 weeks,Telmisartan (3.7mg/kg) was given to positive group after DN model successful for 4 weeks.The rabits were executed at 12w.Daily monitoring of blood glucose,urine protein,Detect the content of malondialdehyde(MDA),nitric oxide(NO),nitric oxide synthetase(NOS) and the activity of superoxide Dismutase(SOD) in kidneys of the executed rabits,observe the effect of Lycium barbarum polysaccharides(LBP) on oxidative stress reaction in renal function of diabetic nephrosisi rabbits.Result:The blood glucose level,urine protein in prevention group were significantly decreased, and the content of SOD was more,MDA was less compared with negative group(P<0.05),the level of NO and NOS were also better than other groups(P<0.05).Conclusion;The effect of Lycium barbarum polysaccharides(LBP) on anti-oxidative stress reaction in renal function of diabetic nephrosisi rabbits probably is one of the mechanism of its therapeutic effects.更多还原