【作者】 李菁菁； 罗琼； 闫俊； 耿天琪； 赵颀涵；

【Author】 LI Jing-jing LUO Qiong YAN Jun GENG Tian-qi ZHAO Qi-han (School of public Health,Wuhan Universitiy Wuhan,430070,China)

【机构】 武汉大学公共卫生学院营养与食品卫生学系；

【摘要】 目的:探讨枸杞多糖(LBP)对糖尿病肾病兔肾脏的保护作用及单核细胞趋化蛋白-1(MCP-1)、细胞间粘附分子-1(ICAM-1)mRNA表达的影响。方法:采用四氧嘧啶(ALX)诱导糖尿病肾病兔动物模型。将20只雄性日本大耳白兔随机分为5组,每组4只,分别为正常对照组、阴性对照组、预防组、治疗组和阳性对照组。预防组于糖尿病(DM)成模后即灌胃LBP(10mg/kg)持续12w,治疗组和阳性组于糖尿病肾病(DN)成模(第8w)后分别灌胃LBP(10mg/kg)和美卡素(3.7mg/kg)持续4w。实验第4w、6w、8w和12w测空腹血糖及24h尿蛋白定量;实验第12w,采集血液标本,测定C反应蛋白(CRP)、尿素氮(BuN)和血肌酐(SCr),RT-PCR方法测定肾皮质MCP-1、ICAM-1mRNA表达。结果:与正常组相比,阴性组大耳白兔24h尿蛋白明显升高(P<0.01),血清中BUN、SCr和CRP含量明显增加(P<0.01),肾皮质MCP-1、ICAM-1mRNA表达明显上升(P<0.01)。预防组大耳白兔24h尿蛋白明显降低,血清中BUN、SCr和CRP明显降低,肾皮质MCP-1、ICAM-1mRNA表达明显下调,与阴性组比较均有显著性差异(P<0.01)。治疗组与阴性组相比,24h尿蛋白明显下降(P<0.05),血清中BUN、CRP明显降低(P<0.01),肾皮质MCP-1、ICAM-1mRNA表达明显降低(P<0.01)。结论:LBP能改善DN兔肾功能,减少MCP-1、ICAM-1mRNA表达,减轻炎症反应,延缓DN的发生发展。更多还原

【Abstract】 Objective:To investigate the effect of Lycium barbarum polysaccharides(LBP) on renal function of diabetic nephrosisi rabbits and expression of MCP-1 mRNA and ICAM-1 mRNA.Methods:Diabetes was induced by injecting alloxan(ALX).Japanese white rabbits were randomly divided into 5 groups:normal control group,negative control group,prevention group,treatment group,positive control group(n = 4 for each group).LBP(10mg/kg) was given to prevention group after DM model successful for 12 weeks and treatment group after DN model successful for 4 weeks,Telmisartan(3.7mg/kg) was given to positive group after DN model successful for 4 weeks.Fasting blood glucose and 24h urine protein were detected at 4w、6w、8w and 12w,and determination of serum c-reactive protein,urea nitrogen and serum creatinine at 12w.The expression of MCP-1mRNA and ICAM-1mRNA were detected by RT-PCR.Result;24h urine protein was significantlyincreased in negative group compared with normal group (P<0.01),and BUN.,SCr and CRP in serum were increased(P<0.01).The expression of MCP-1mRNA and ICAM-1mRNA were increased(P<0.01).Compared with negative group,24h urine protein,the serum of BUN、SCr and CRP,the expression of MCP-1mRNA and ICAM-1mRNA were significantly decreased in prevention group and treatment group(P<0.05,P<0.01).Conclusion;LBP can improve renal function of diabetic nephrosisi rabbits, reduce the expression of MCP-1mRNA and ICAM-1mRNA and delay the development of DN.更多还原