【作者】 李蕾； 王茵； 武洁姝； 朱染枫； 赵显峰； 汪之顼； 荫士安；

【Author】 LI Lei,WANG Yin,WU Jieshu,ZHU Ranfeng,et al. National Institute for Nutrition and Food Safety,Chinese Centre for Disease Control and Prevention,Beijing 100050,China

【机构】 中国疾病预防控制中心营养与食品安全所； 浙江省医学科学院保健食品研究所； 青岛大学医学院营养研究所；

【摘要】 目的确定菠菜及纯品油胶囊中β-胡萝卜素在学龄儿童体内的转化效率。方法选取某农村小学7～9岁儿童32名,随机分为2组,每组16名,维生素A正常和缺乏者各8名,前7天每天午饭及晚饭两组分别补充氘标记菠菜5g(含²H₁₀-β-胡萝卜素230μg)及纯品β-胡萝卜素油胶囊（含²H₈-β-胡萝卜素200μg）,早饭两组均服用¹³C₁₀醋酸视黄醇100μg。在实验的第3、7、8、22及28天分别采集静脉血3ml,用HPLC及GC-MS测定血清中的视黄醇浓度及标记同位素的丰度,计算菠菜及纯品油胶囊中β-胡萝卜素的转化效率。结果β-胡萝卜素油胶囊组维生素A正常和边缘缺乏的儿童β-胡萝卜素的转化率分别为2.9.1和3.2.1（质量比）;菠菜组分别为10.1;1和10.3.1。结论纯品油胶囊中β-胡萝卜素的转化效率明显高于菠菜;不同维生素A营养状况的儿童β-胡萝卜素的转化效率无明显差异。更多还原

【Abstract】 Objective To determine the bioefficacy of pureβ- carotene andβ- carotene from spinach to vitamin A in schoolciiildren of different vitamin A status.Methods Thirty two schoolchildren aged 7- 9 yeais old were selected and then divided into two groups randomly.Each group included 16 subjects,of which the vitamin A level of 8 subjects was normal, the other 8 subjects suffered from vitamin A deficiency.In the first 7 days,one group was given 5 gram spinach (containing 230μg ²H_l0-β- carotene) before lunch and dinner and the other group was given pureβ-carotene capsule （containing 200μgβ- carotene）.Before breakfast,both groups were given l00μg ¹³C₁₀ retinal acetate capsule.Blood was drawn on the 3,7,8,22 and 28 day.HPLC and GC-MS were used to determine the retinol concentration and enrichment of isotope and then calculate the bioefficacy of pureβ- carotene andβ- carotene from spinach to vitamin A.Results The conversion factors of pureβ- carotene to vitamin A in oil capsule were:2 9:1 for the children with normal vitamin A status, 3.2 1 for tliose suffering from marginal vitamin A deficiency.The conversion factors of spinachβ- carotene to vitamin A were 10.1:1 and 10.3:1 for the children with normal and low vitamin A status,respectively.Conclusion The vitamin A conversion bioefficacy of pureβ- carotene from oil capsule is much higher than that of spinach.There is no difference ofβ- carotene bioefficacy to vitamin A for children with different vitamin A status.更多还原