【作者】 徐海滨； 张晓霞； 李芳； 王国英； 刘允军；

【Author】 XU Haibin,ZHANG Xiaoxia,LI Fang,WANG Guoying et al. Institute of Nutrition and Food Safety,Chinese Center for Disease Control and Prevention,Beijing 100021,China

【机构】 中国疾病预防控制中心营养与食品安全所； 中国农业大学生物技术中心；

【摘要】 目的以Cry1 Ie基因表达蛋白为模式蛋白,建立外源基因表达蛋白的技术平台。方法利用大肠杆菌系统高效表达Cry1 Ie蛋白,并采用SDS-PAGE技术分离纯化,通过生物学活性和免疫反应性2项指标检测了大肠杆菌表达蛋白与转基因玉米中蛋白的等同性。结果大肠杆菌表达系统可以产生大量的Cry1 Ie蛋白,所获得的蛋白与转Cry1 Ie玉米表达的Cry1 Ie蛋白有相同的免疫反应性和生物活性。结论大肠杆菌表达系统可以作为Cry1 Ie外源基因蛋白表达技术平台用于转基因植物食用安全性的初步研究。更多还原

【Abstract】 Objective In this study,Crylle expressed protein in the transgenic Crylle maize was used as a model protein to establish a technical platform for the safety assessment of other genetically modified plants.Methods Bacillus coli expression system was used to express Cryl Ie protein and then the expressed protein was purified by SDS PAGE.The equivalence of Crylle proteins expressed in Bacillus cdi and in transgenic Crylle maize was analyzed by their immunorecognition and bioactivity.Results Crylle protein was highly expressed in Bacillus coli and could be segregated and purified by SDS-PAGE.Cryl Ie expressed protein in Bacillus coli is substantially equivalent to that of maize genetically modified with Crylle in tests of immunorecognition western blot and bioactivity in target pest.Conclusion The established technical platform of external Crylle expressed protein can be applied to assess the food safety of genetically modified plants.更多还原