【作者】 孙忠； 吴蕴棠； 车素萍； 王夏； 郭刚；

【Author】 Sun Zhong Wu Yun-tang,Che Su-ping,Wang Xia,et al. College of Public Health,Tianjin Medical Univeisity,Tianjin 300070,China

【机构】 天津医科大学公共卫生学院； 中国科学院微生物研究所；

【摘要】 目的研究补铬对糖尿病大鼠骨骼肌代谢相关基因表达的影响。方法对前期研究分离到的与补铬有关的糖尿病大鼠差异显示的基因片段进行克隆、测序,并进行同源性分析。根据待检测的基因序列进行引物设计,进行RT-PCR检测。用激光密度扫描仪进行光密度扫描分析,以目的基因与参考基因的灰度比值反映其表达水平。结果经过克隆、测序、同源性比较,差显片段Cr-5的碱基序列与IRS-1同源性为100%。糖尿病对照组IRS-1 mRNA表达量(0.791±0.038)低于正常对照组(0.892±0.053,P<0.05),糖尿病补铬组IRS-1 mRNA的表达量(0.822±0.066)与糖尿病对照组相比有所恢复,但尚未恢复到正常水平(P>0.05)。结论补铬对糖尿病大鼠骨骼肌IRS-1 mRNA表达有上调趋势,Cr-5将作为候选基因有待于进一步研究。更多还原

【Abstract】 Objective To study the effects of chromium supplementation on related gene expression to metabolism of skeletal muscles in diabetic rats.Methods cDNA fragments from the our related research were cloned,sequenced and its comparalarity analysis has been done.The RT- PCR were made using the primers designed according to the sequence of cDNA.Results The identities between the sequence of Cr 5 and IRS 1 is 100%.The expression level of DM group (0.791±0.038) is obviously lower than those of normal group(0.892±0.053,P<0.05).And The expression level of DM+ Cr group(0.822±0.066) have some improvement compare to those of DM group(P>0.05).Conclusion There is a tendency that Chromium supplementation can increase the expression level of IRS-1 mRNA of skeletal muscles in diabetic rats.It will be studied as a candidate gene in future research.更多还原