【作者】 李珂； 姚平； 周绍良； 宋方方； 章锡平； 孙秀发； 刘烈刚；

【Author】 Li Ke,Yao Ping,Zhou Shao-liang,Song Fang-fang,et al. Department of Nutrition and Food Hygiene,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

【机构】 华中科技大学同济医学院公共卫生学院营养与食品卫生学系；

【摘要】 目的研究银杏叶提取物(EGB)对酒精所致大鼠睾丸氧化损伤的预防保护作用。方法雄性SD大鼠30%酒精灌胃(2.37g/kg bw)前,EGB采用分组(低剂量组48μg/g bw和高剂量组96μg/g bw)预防性给药的方法。于实验90 d检测大鼠睾丸组织中超氧化物歧化酶(SOD)、谷胱甘肽转移酶(GST)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、和谷胱甘肽(GSH)、活性氧(ROS)以及丙二醛(MDA)的含量。通过亚细胞分离方法提取大鼠睾丸微粒体,测定微粒体血红素氧化酶-1(HO-1)活性。采用RT-PCR检测睾丸组织中血红素氧化酶-1(HO-1)mRNA表达水平。结果慢性酒精摄入90d后,EGB组睾丸匀浆ROS、MDA较酒精组有明显降低(P<0.05);相反,GST、G-Px、CAT、SOD、HO-1活性以及GSH均有显著性升高(P<0.05);EGB诱导HO-1高表达。结论 :长期慢性酒精摄入引起大鼠睾丸氧化损伤。EGB可作为1种预防性的抗氧化剂减轻这种氧化损伤;其机制可能与诱导HO-1高表达,清除自由基,抑制脂质过氧化反应有关。更多还原

【Abstract】 Objective This study was designed to investigate whether EGB protect against et hand-induced oxidative injury in rat testes.Methods The rats were pretrested will EGB(4.8,9.6mg/l00g bw per day) before 30%ethanol administration(2 37g/kg bw by gastric incubation).Ninety days later,the rats were killed in order to measure the activities of Superoxide Disnutase(SOD),Glutathione Reductase(GST),Glutathione Peroxidase(GSH-Px),Catalase (CAT) and the contents of Glutathione(GSH),Reactive Oxygen Species(ROS),Malondialdehyde(MDA) in rat testes.The microsomal fractions were obtained by the method of differential centrifugation,and HO l(Heme Oxygenase 1) activity in testicular microsomal was measured.To examine the expression of HO-1 mRNA by reverse transcription polymerase chain reaction(RT-PCR).Results Compared with etlianol group,ROS,and MDA contents significantly decreased in EGB group testes,respectively(P<0.05).GST,GSH-Px,CAT,SOD activities,and GSH content in EGB group testes elevated markedly(P<0.05).EGB could enhance HO- lmRNA expression remarkably.Conclusion It is suggested that EGB as a preventive antioxidant can protect against etlianol- induced testicular injury,which may be associated with HO-1 activity enhancement,free radicals elimination,lipid per oxidation reaction inhibition.更多还原