【作者】 杨年红； 王重建； 许明佳； 胡学锋； 郝丽萍； 刘烈刚； 孙秀发；

【Author】 Yang Nian-hong,Wang Chong-jian,Xu Ming-jia,Hu Xue-feng,et al. Department of Nutrition and Food Hygiene,School of Public Health,Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030,China

【机构】 华中科技大学同济医学院公共卫生学院营养与食品卫生系；

【摘要】 目的探讨白色脂肪组织UOPs和PPARγ2基因在高脂饮食诱导大鼠肥胖抵抗中的作用。方法 36只雌性SD大鼠,按体重随机分为高脂实验组和基础对照组,分别给予高脂饲料和基础饲料13周。实验结束时,根据休重将高脂组大鼠分为饮食诱导肥胖(DIO)和饮食诱导肥胖抵抗(DIO-R)大鼠,比较各组大鼠休重、脂肪湿重、脂体比、空腹血糖(FBG)及血脂谱;RT-PCR法测定白色脂肪组织UCP2、UCP3及PPARγ2 mRNA的表达水平。结果 DIOR及DIO大鼠脂体比、TC、LDL-C、TG等指标均显著高于对照组相.但DIO-R组体重、脂肪湿重、脂体比、TG显著低于DIO组(P<0.05);DIO-R大鼠UCP2和UCP3 mRNA的表达水平高于DIO大鼠和对照组(P<0.01).PPARγ2 mRNA的表达水下低于DIO大鼠(P<0.01),高于对照组(P<0.01)。结论高脂饮食诱导大鼠发生肥胖抵抗与白色脂肪组UCP2、UCP3高表达及PPARγ2 mRNA表达下降密切相关。更多还原

【Abstract】 Objective To explore the expression of uncoupling proteins(UCP) and peroxisome proliferated activated receptorγ2(PPARγ2) mRNA in white adipose of high-fat diet induced obesity resistant rats.Methods Thirty-six female SD rats were randomly divided into higlrfat diet group(n - 27) and chow fed control group(n - 9),and given either high-fat diet or chow for thirteen weeks.Then the high-fat diet group was subdivided into Dietary Induced Obesity(DIO) and Dietary Induced Obesity Resistant(DIO-R) group according to the final body weight.Basic parameters,fasting blood glucose(EBG),blood lipid levels were measured and compared.Reverse transcription-polymerase chain reaction(RTPCR) was used to measure the expression of UCP2,UCP3,and PPARγ2 mRNA in white adipose.Results Adipose deposits,body fat percent,TC,and TG conceration of both DIO-R and DIO groups were significantly higher than those in control group,while body weight,body fat percent and TG level were significantly lower in DIO-R than DIO rats.The expression of UCP2 and UCP3 mRNA in white adipose of DIO-R Tats were significantly higher than those of DIO and control group.The expression of PPARγ2 mRNA in white adipose of DIO-R group was significantly lower than that in DIO group. Conclusion Higlrfat diet induced obesity resistance were closely associated with the increased UCP2,UCP3,and decreased PPARγ2 mRNA in white adipose.更多还原