【作者】 陈祥贵； 王瑞淑； 邓茂先； 冉新泽； 粟永萍；

【Author】 Chen Xianggui,Wang Ruishu,Deng Maoxian,Ran Xinze,et al. Department of Food Engineering,Sichuan University of Science and Technology,Chengdu 610039,China

【机构】 四川工业学院食品工程系； 四川大学华西公共卫生学院； 第三军医大学预防医学系；

【摘要】 通过对体外培养的大鼠小肠上皮细胞系IEC-6添加核苷酸,探讨四种单核苷酸及其按母乳组成模式配制的核苷酸混合物对肠上皮细胞增殖和损伤后迁移的影响。采用MTT法测定核苷酸对细胞增殖的浓度-效应和核苷酸之间对细胞增殖效应的相互作用;采用IEC-6单层肠上皮损伤重建模型,通过免疫组化检测TGFβ的表达测定细胞损伤后迁移。结果显示嘌呤核苷酸AMP和GMP分别在30μmol/L和150μmol/L及以上浓度能显著抑制IEC-6细胞的增殖并表现浓度依赖,嘧啶核苷酸CMP仅在极高浓度(1440μmol/L)时对细胞增殖有抑制作用,UMP和核苷酸混合物在受试浓度范围内未见明显作用,但是,CMP和UMP能解除嘌呤核苷酸AMP和GMP对细胞增殖的抑制。180μmol/L核苷酸混合物能促进细胞损伤后迁移,但不诱导TGFβ的表达。提示嘌呤核苷酸抑制肠上皮细胞增殖,而嘧啶核苷酸能解除嘌呤核苷酸的抑制作用。核苷酸混合物能通过非TGFβ依赖的途径促进肠上皮细胞的损伤后迁移。更多还原

【Abstract】 The effect of exogenous nucleotides on proliferation and migration of a normal rat small intestinal epithelial cell line IEG-6 is studied.The concentration-effects as well as interaction of exogenous nucleotides on proliferation of IEG- 6 are measured by MTT.M igration of IEG-6 after wounded is determined by an in vitro model of intestinal epithelial restitution of IEG-6 monolayer.Expression of TGFβis detected by immnohistochemistry.The results show AMP and GMP remarkably inhibit proliferation of IEG-6 in concentration-dependent manner respectively at 30μmol/L or 150μmol/L and more. CMP,UMP and nucleotides mixture cannot enhance or inhibit the growth with the exception of inhibition of CMP on proliferation at very high concentration(1440μmol/L).In contrast,CMP,especially UMP,can remarkably abolish the proliferation -inhibiting effects of AMP or GMP on the cell,when AMP or GMP is supplemented.Nucleotides mixture significantly facilitate migration of IEG-6 after wounded but fail to promote the expression of TGFP.It is concluded that purine nucleotides inhibits proliferation of IEC-6.Pyrimidine nucleotides can abolish the inhibitive effects of purine nucleotides,and Nucleotides mixture promotes migration of IEG-6 after wounded by a TGEβ-independented way.更多还原