【作者】 付荣霞； 孙长颢； 孙文广； 唐亚兰； 刘荣； 王舒然；

【Author】 Fu Rongxia,Sun Qianghao,Sun Wenguang,Tang Yalan,et al. College of Public Health of Harbin Medical University,Harbin 150001,China

【机构】 哈尔滨医科大学公共卫生学院；

【摘要】 为探讨不同饲料构成对大鼠褐色脂肪组织解偶联蛋白-1基因表达的影响,利用不同的饲料(包括基础饲料、高蛋白、高脂肪、高能1与高能2饲料)喂养雄性Wistar大鼠2个月,观察不同饲料构成对大鼠体重、脂体比的影响,并应用RT-PCR、蛋白印迹法测定大鼠褐色脂肪组织UCP1 mRNA与蛋白含量。结果高能饲料1组与高能饲料2组的体重、脂体比均显著高于基础组、高蛋白组与高脂肪组(P<0.05),而后三者的体重、脂体比无显著性差异(P>0.05)。高能饲料1组与高能饲料2组UCP1基因表达增强,而能量相同的基础组、高蛋白组与高脂肪组没有明显差异。说明能量能诱导大鼠褐色脂肪组织UCP1基因表达。更多还原

【Abstract】 In order to explore the effect of different diet composition on uncoupling proteins 1(UCP1) gene expression in brown adipose tissue,male Wistar rats are fed with different diets(basic diet,high protein diet,high fat diet,high calorie diet 1 and 2) for 2 months.The effects of different diets on weiglt and the ratio of fat mass to body weigh are observed. Reverse transcription-polymerase chain reaction and western blotting technique are used to measure UCPl mRNA and protein content of rat brown adipose tissue(BAT).The results show that body weiglt and the ratio of fat mass to body weight in high calorie 1 and 2 diet groups are obviously higher than those in groups of basic diet,high protein,and high fat diet(P< 0.05),but there is no significant difference among the last three groups(P> 0.05).UCPl gene expression increases in both high calorie diet 1 group and 2 group,but it is not significantly different among basic diet,high protein and high fat diet groups with equal energy,indicating that energy can induce UCPl gene expression of rat BAT.更多还原