【作者】 王东坤； 黄云超； 陈安宁； 雷玉洁； 陈小波；

【Author】 WANG Dongkun;HUANG Yunchao;CHEN Anning;LEI Yujie;CHEN Xiaobo;Third Affiliated Hospital of Kunming Medical College(Tumor Hospital of Yunnan Province);

【机构】 昆明医学院第三附属医院(云南省肿瘤医院)胸外科；

【摘要】 目的:采用免疫抑制的方法,建立树鼩的肺癌模型。在此基础上,以医源性表皮葡萄球菌临床分离株为研究对象,通过动物实验,探讨生物材料植入对肿瘤动物的影响,为防治临床中以生物材料为中心的感染(Biomaterial Centered Infection,BCI)提供有益帮助,从而为肿瘤的进一步治疗创造条件。方法:使用不同的免疫抑制方法,建立树鼩肿瘤模型。健康成年树鼩25只,雌雄各半。随机分组,分别采用不同的处理方法。A组为阴性对照组,仅给予生理盐水处理。B、C、D、E组为实验组,B、C、D组依次给予6Gy、8Gy、10Gy加速器电子线照射处理,E组给予环磷酰胺免疫抑制处理。之后各组均接种恶性肿瘤细胞悬液。观察树鼩的存活及成瘤情况,以筛选出树鼩肺癌模型的最佳实验条件。结果:经加速器电子线照射免疫抑制处理的树鼩,射线照射后活动减少、行动迟缓、精神萎靡,进食减少,甚至拒食。体重减轻明显。接种恶性肿瘤细胞悬液后。天可见肿瘤生长,肿瘤生长迅速,3周时肿瘤肿瘤结节最大,此后肿瘤生长缓慢,部分肿瘤结节甚至缩小。血细胞分析提示,射线照射后,树鼩出现明显骨髓抑制,1周后血象开始恢复,第3周时,血细胞分析白细胞计数接近正常,P>0.05,提示免疫基本恢复。使用环磷酰胺免疫抑制处理的树鼩,与经加速器电子线照射的树鼩表现类似,动物死亡率高,实验期间未见肿瘤结节生长。对照组树鼩实验期间全部存活,未见肿瘤结节生长。建立树鼩肿瘤模型的最佳条件为8Gy的加速器电子线照射,接种的宣威女性肺腺癌细胞(XWLC-05)量为5×106。其生存率为63.89%,成瘤率为61.11%。结论:使用为8Gy的加速器电子线照射,接种5×106的宣威女性肺腺癌细胞(XWLC-05),可以建立树鼩肿瘤模型,其生存率为63.89%,成瘤率为61.11%。更多还原

【Abstract】 Objective: Using the immune suppression to establish lung cancer model in tree shrew. Method: 25 healthy adult tree shrew, half male and half female, Random grouping. Group A was negative in the control group, only received saline treatment. B, C, D, E groups, B, C, D, 8Gy, 6Gy groups were given 10Gy electron accelerator radiation treatment, E group were given cyclophosphamide immunosuppression treatment. After the rats were implanted with tumor cell suspension. To observe the survival and growth of tree shrews, to select the best experimental conditions of tree shrew lung cancer model. Result: Use immunosuppressive treatment of tree shrew, and treated by electron accelerator radiation tree shrew like animal, high mortality during the experiment, no tumor growth. The control group during the experiment in tree shrews survived, no tumor growth. The best conditions for the establishment of tree shrew tumor model for the accelerator line 8Gy irradiation, inoculation of Xuanwei female lung adenocarcinoma cell(XWLC-05) is 5 × 106. The survival rate was 63.89%, tumor formation rate is 61.11%. Conclusion: Use the accelerator line 8Gy irradiation, with 5 × 106 Xuanwei female lung adenocarcinoma cells(XWLC-05), may establish the tree shrew tumor model, the survival rate was 63.89%, tumor formation rate is 61.11%.更多还原