【作者】 贺金华； 蔡晓翠； 何江； 毛艳； 杨伟俊； 戎晓娟；

【Author】 HE Jinhua 1,2 ,CAI Xiaocui 1 ,He Jiang 1 ,MAO Yan 1,2 ,YANG Weijun 1 ,RONG Xiaojuan 1 (1. Xinjiang Institute of Materia Medica, Urumqi 830004, China;2. Key Laboratory of Xinjiang Uygur Medicine, Urumqi 830004, China )

【机构】 新疆维吾尔自治区药物研究所； 新疆维吾尔药重点实验室；

【摘要】 目的:建立高效液相色谱法(HPLC)测定对叶大戟草中槲皮素和山柰酚含量的方法。方法:采用Hypersil ODS-2C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸溶液(52:48);检测波长为360nm;流速为1.0mL/min;柱温为30℃。结果:槲皮素和山柰酚对照品浓度分别在5.2～156.0μg/ml(r=0.9999)和3.07～92.1μg/ml(r=0.9997)范围内,与峰面积线性关系良好,平均回收率分别为101.39%(RSD=1.60%)和101.80%(RSD=1.29%)。结论:高效液相色谱法操作简单快速,重现性好,可用于对叶大戟草中槲皮素和山柰酚的含量的测定。更多还原

【Abstract】 Objective: To establish a method for the determination of quercetin and kaempferide in Euphorbia sororia A. Schrenk. Methods: The RP-HPLC system consisting of Hypersil ODS-2 C 18 column(250 mm×4.6 mm I.D.,S-5 μm) and a mixture of methanol solution and 0.4% phosphonic acid solution(52:48)as the mobile phase was adopted; the detection wavelength was 360 nm; the flow rate was set at 1.0 mL / min and the column temperature was 30 ℃. Results: The linear response ranges of quercetin and kaempferide were 5.2～166.0 μg /ml(r=0.9999)and 3.07～92.1 μg /ml(r=0.9997), repectively. The average recoveries (n=6) were 101.39% (RSD=1.60%) and 101.80%(RSD=1.29%), respectively. Conclusion: The method is quick, simple and reproducible for the determination of quercetin and kaempferide in Euphorbia sororia A. Schrenk. simultaneously.更多还原