【作者】 陈烟兰； 黄婧； 曾如意； 谢丽华； 杨民和；

【Author】 CHEN Yanlan 1 , HUANG Jing 1 , ZENG Ruyi 1 , XIE Lihua 1 , YANG Minhe 1,2 (1.College of Life Science, Fujian Normal University, Fuzhou Fujian, 350108, China;2.Engineering Research Center of Industrial Microbiology Affiliated to Ministry of Education, Fujian Normal University, Fuzhou Fujian,350108, China)

【机构】 福建师范大学生命科学学院； 福建师范大学工业微生物发酵技术国家地方联合工程研究中心；

【摘要】 儿茶素是茶叶中主要的生化成分,也是茶叶主要的生理活性物质。内生真菌生长于植物体内,在长期的共生进化过程中,与宿主植物构建密切的生理和代谢关系。本研究以茶多酚为唯一碳源,通过富集培养获得23株能分解利用茶多酚的茶树内生真菌。茶多酚能影响茶树内生真菌的生长,在PDA培养基中,随着添加茶多酚浓度的提高,对芒果球座菌的生长的抑制作用逐渐明显;供试的3种茶树内生真菌均能不同程度地产生纤维素酶和脂肪酶,但蛋白酶、淀粉酶和木聚糖酶的表现不尽相同;在以单宁为唯一碳源的鉴别培养基中,供试的15个内生真菌菌株均能生长,除1个菌株外,其余14个菌株生长良好;供试的15个内生真菌菌株均能不同程度地产生单宁酶。在摇瓶培养条件下,芒果球座菌可以降解儿茶素,并产生新的化合物。本研究的初步结果表明:茶树内生真菌在茶叶成分的生物转化中能够发挥一定的作用。更多还原

【Abstract】 Catechins are the most prominent characteristic and functional compounds of tea. Fungal endophytes are microbes that colonize living, internal tissues of plants, without causing any immediate negative effects to their host plants. In this study, 23 isolates were isolated from leaf segments by using tea polyphenols as the sole carbon source in culture media. Results showed that tea polyphenols inhibited the mycelium growth of endophytic fungi isolated from tea plants. Mycelium growth of Guignardia mangiferae was severely affected as the concentration of tea polyphenols in media was increased. Three endophytes were found to produce degrading enzymes like cellulose and lipase. And some of the selected endophytes produced protease, amylase and xylanase. In agar media using tannin as the sole carbon sources, 14 out of 15 tested isolates of endophytic fungi grew well. 15 fungal isolates were tested for their ability to produce extracellular tannin acyl hydrolase and all isolates showed the positive results. The preliminary results also indicated that one strain of Guignardia mangiferae degraded catechin components and transformed them into new compounds.更多还原