【作者】 李清靖； 李巧霞； 单保恩； 檀碧波； 刘羽； 赵雪峰；

【Author】 LI Qingjing,LI Qiaoxia,SHAN Baoen,TAN Bibo,LIU Yu,ZHAO Xuefeng(Research center,The fourth hospital of Hebei medical university,Shijiazhuang,050011,China)

【机构】 河北医科大学第四医院科研中心；

【摘要】 目的:Th17/Treg细胞在免疫调节方面发挥重要的作用,它们之间的失衡可能会导致自身免疫疾病和过敏反应的发生。在癌症中关于Th17/Treg的报道还很有限。本研究的目的是检测Th17和Treg细胞在胃癌患者外周血分布,探讨Th17/Treg细胞动态平衡与胃癌发生发展的关系。方法:收集45例胃癌患者和20例健康体检者外周血,分离单个核细胞(PBMC),经采用流式细胞分析技术检测CD4,CD25,IL-17,Foxp3的表达。产生IL-17的CD4+T认为是Th17细胞和CD4+CD25+Foxp3+细胞为Treg细胞,检测Th17和Treg细胞的数量和比例。结果:与健康对照组相比,胃癌患者外周血中:Th17细胞比例显著增多(1.63±0.10%vs 6.67±0.77%,P<0.01),并与患者临床分期(P=0.002)、淋巴结转移(P=0.017)有关,与性别、年龄、肿瘤分化程度、肿瘤大小、侵润深度、血管侵袭、脉管瘤栓、术前抗原无关(P>0.05);Treg细胞比例明显增加(10.34±1.13%vs 4.79±0.18%,P<0.01),并与患者临床分期(P=0.034)、肿瘤分化(P=0.015)有关,与性别、年龄、肿瘤大小、侵润深度、淋巴结转移、血管侵袭、脉管瘤栓、术前抗原无关(P>0.05);Th17/Treg细胞比例明显增加(0.65±0.05 vs 0.34±0.03,P<0.01),并与患者淋巴结转移有关(P=0.036),与性别、年龄、临床分期、肿瘤分化程度、肿瘤大小、侵润深度、血管侵袭、脉管瘤栓、术前抗原无关(P>0.05)。采用Pearson相关分析显示:健康体检者外周血中Th17和Treg细胞呈正相关(R=0.64,P=0.003),但在胃癌患者中两者无相关性(R=0.12,P=0.49)。结论:胃癌患者Th17、Treg细胞及Th17/Treg细胞比值明显增高,并与患者TNM分期、淋巴结转移、肿瘤分化程度有关,提示胃癌患者存在Th17/Treg细胞免疫平衡失调,这种失调可能促进了胃癌的进展,并影响患者预后。更多还原

【Abstract】 Objective: Th17/Treg was reported to play critical roles in immunoregulation,and its imbalance may lead to autoimmune diseases and allergic reactions.Information on Th17/Treg in cancer is still limited.The purpose of this study was to detect the distribution of Th17 and Treg cells in peripheral bloods,to investigate the possible involvement of Th17/Treg imbalance in gastric cancer.Methods: Peripheral blood was collected from 45 gastric cancer patients and 20 healthy volunteers.Peripheral blood mononuclear cells(PBMCs) were obtained by Ficoll-Hypaque density centrifugation of peripheral blood.We examined the expression of CD4,CD25,IL-17 and Foxp3 by flow cytometry.Interleukin(IL)-17-producing CD4+ cells as Th17 and CD4+CD25+Foxp3+ cells as Treg were expressed as a percentage of the total CD4+ cells.Results: Flow cytometry analysis results showed that,compared with healthy controls,Th17 cell proportion increases significantly in peripheral blood in patients with gastric carcinoma(1.63± 0.10% vs 6.67 ±0.77,P<0.01),and correlated with clinical stage(P=0.002),lymph node metastasis(P=0.017),but had no correlation with patient gender,age,tumor differentiation degree,tumor size,invasion,vascular invasion,Intravascular cancer embolus,preoperative unrelated antigens(P>0.05);Treg number had a significant increase in patients,and was associated with gastric cancer clinical staging(P=0.034),tumor differentiation(P=0.015),but had no association with gender,age,tumor size,depth of invasion,lymph node metastasis,vascular invasion,Intravascular cancer embolus,preoperative unrelated antigens(P>0.05);The ratio of Th17/Treg in patients with gastric cancer,(0.65±0.05 vs 0.34±0.03,P<0.01) was significantly increased(P<0.01),and correlated with lymph node metastasis(P=0.036),and had no correlation with patient sex,age,clinical stage,tumor differentiation,tumor size,invasion,vascular invasion,Intravascular cancer embolus,preoperative unrelated antigens(P>0.05).Th17 cells and Treg cells were positively correlated(R=0.64,P=0.003) in peripheral blood of healthy group,but no correlation in the patients with gastric cancer(R=0.12,P=0.49).Conclusions: Th17 cells,Treg cells,and the radio of Th17/Treg increased in patients with gastric cancer,correlated with the patients with TNM staging,lymph node metastasis,tumor differentiation,suggesting that Th17/Treg immune imbalance was presenced in patients with gastric cancer,this imbalance may promote the progression of gastric cancer and affect the prognosis of the patients.更多还原