【作者】 尹艳娥； 沈新强； 晁敏；

【Author】 YIN Yane, SHEN Xinqiang, CHAO Min (East China Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Key and Open Laboratory of Marine and Estuarine Fishery, Ministry of Agriculture, Shanghai 200090, China)

【机构】 农业部海洋与河口渔业重点开放实验室中国水产科学研究院东海水产研究所；

【摘要】 本研究从浙江奉化市池塘的底泥经过反复地培养、驯化,从中筛选、分离出了反硝化细菌,在模拟实验条件下,研究了对不同浓度的硝酸盐氮和亚硝酸盐氮的去除情况,讨论了反硝化菌种的生长情况。研究结果表明,分离出的反硝化细菌能有效去除硝酸盐氮和亚硝酸盐氮,在初始浓度为1mg/L的模拟池塘中,随着实验的进行,对污染物的去除效果逐渐提高,三天内硝酸盐氮去除率就达到了95.8%,亚硝酸盐氮的去除率达到了90.2%;在25mg/L的模拟池塘中,第六天硝酸盐氮和亚硝酸盐的去除率达到了93.8%和87.8%;在50mg/L的模拟池塘中,第六天硝酸盐氮和亚硝酸盐的去除率达到了89.7%和78.7%。而且在三个不同浓度的模拟池塘中,反硝化菌对硝酸盐氮的去除效果略优于亚硝酸盐氮。菌种地生长情况与硝酸盐氮和亚硝酸盐氮的浓度成反比,即浓度越高菌种生长情况越差。更多还原

【Abstract】 By repeatedly cultivating and domesticating, denitrifying bacteria were screened from aquaculture pond sediment in Fenghua Zhejiang.In the stimulant experiment, the removal efficiency of nitrate-N and nitrite-N and growth of denitrifying bacteria were researched. The result showed that the removal efficiency gradually improved with days. The removal efficiency of nitrate-N and nitrite-N reached 95.8% and 90.2% within three days when initial concentration are both 1mg/L.About 93.8% and 87.8% of nitrate-N and nitrite-N were removed by denitrifying bacteria on the sixth day when the initial concentration are both 25mg/L. About 89.7% and 78.7% of nitrate-N and nitrite-N were removed by denitrifying bacteria on the sixth day when the initial concentration are both 50mg/L. In the three different aquaculture ponds, the removal efficiencies of nitrate-N were better than nitrite-N. It was found that there was correlation between the concentration of nitrate-N, nitrite-N and the number of denitrifying bacteria in the water. The concentration of nitrate-N, nitrite-N were more, the number of denitrifying bacteria were more.更多还原