【作者】 王斌； 刘双凤； 袁甜； 姜白洁； 程振远；

【Author】 WANG Bin, LIU Shuangfeng, YUAN Tian, JIANG Baijie, CHENG Zhenyuan (Key Laboriatory of Mariculture Biotechnology Agriculture Ministry, Dalian Fisheries University, Dalian 116023, Liaoning, China)

【机构】 大连水产学院农业部海洋水产增养殖与生物技术重点开放实验室；

【摘要】 用分离自患病大菱鲆(Scophthalmus maximus)的迟钝爱德华氏菌(Edwardsiella tarda)L-49231菌株接种体外培养细胞(HaCaT细胞),观察其对细胞的粘附性和侵袭性,并研究了不同孵育时间对HaCaT细胞粘附性及侵袭性的影响,不同处理因子处理细菌对细胞的粘附性和侵袭性的影响。结果表明:L-49231菌株能粘附HaCaT细胞,对HaCaT细胞具低度侵袭力,并且细菌粘附细胞与侵入细胞的数量随作用时间的增加而增加。分别用甲醛、戊二醛、胰蛋白酶、盐酸处理L-49231菌体,细菌粘附力及侵袭力均有所下降;用蔗糖、甘露糖处理菌体,细菌的粘附力变化不大,用葡萄糖处理,能够促进细胞对细胞的粘附作用,侵袭实验结果表明,用甘露糖处理菌体,侵袭力变化不大,用蔗糖、葡萄糖处理菌体,能促进细菌对细胞的侵袭作用;用胰酶处理菌体,能抑制细菌对细胞的侵袭作用。用自制抗体处理L-49231菌体,细菌粘附力及侵袭力均有所下降。更多还原

【Abstract】 The adhesive speciality and invasion speciality of the L-49231 strain that was isolated from diseased Scophthalmus maximus and indentified to be Edwardsiella tarda were observed with being inoculated in 24-well cell culture plate with HaCaT cell. The influence of different action time and different factors to adhesive speciality and invasion speciality of the L-49231 strain were studied . The rusults indicate : L-49231 strain can adhere HaCaT cell and invade into it after 2 hours. The quantity of bacteria adhered and invaded to cell increased with the prolonged action time.The adhesive and invasion capability of L-49231 strain declined after treated with Formaldehyde, Glutaraldehyde, Hydrochloric acid, Trypsin; adhesive capability varied little in disposal of Sucrose and D-mannose,but increased with Glucose. The effect to invasion capability indicate: the rate of invasion to cell to rise when the strain L-49231 was dealed with Glucose and D-mannose; when dealed with Sucrose, the rate of invasion to cell was partially restrained, when dealed with antibody, both its adhesion and invasion ability declined distinctly.更多还原