【作者】 李厚勇； 颜冬英； 戈扬； 刘永霞； 王蕊； 张振铃；

【Author】 LI Houyong1, YAN Dongying2, GE Yang1, LIU Yongxia1, WANG Rui1, ZHANG Zhenling1 (1.Shandong Academy of Occupational Health and Occupational Medicine,Jinan 250062, China 2.Weifang Medical University, Weifang 261053, Shandong, China)

【机构】 山东省职业卫生与职业病防治研究院； 潍坊医学院；

【摘要】 目的:探讨氰菌胺原药的致突变作用。方法:小鼠骨髓嗜多染红细胞微核试验分别以1000、500、250mg/kg剂量经口给药2天,小鼠睾丸初级精母细胞染色体畸变试验分别以2000、1000、500mg/kg剂量经口给药5天,观察骨髓嗜多染红细胞微核率及睾丸初级精母细胞染色体畸变率。Ames试验(活化和非活化),选用TA97、TA98、TA100和TA102四个菌株,试验剂量为5000、1000、200、40μg/皿,观察各菌株的回变菌落数。结果:小鼠骨髓嗜多染红细胞微核率及小鼠睾丸精母细胞染色体畸变率与阴性对照组比较,均无显著性差异(P>0.05)。Ames试验(活化和非活化),氰菌胺原药各剂量组的回变菌落数均未超过自发回变菌落数的两倍,亦未见剂量-反应关系,Ames试验结果为阴性。结论:氰菌胺原药无致突变作用。更多还原

【Abstract】 Objective:To study the mutagenicity effects of Fenoxanil. Methods:The mice were gavaged Fenoxanil at dose of 1000、500、250m g·kg-1·d-1 for 2 days micronucleus of bone marrow tested. The mice were gavaged Fenoxanil at dose of 2000、1000、500m g·kg-1·d-1 for 5 days Chromosome aberration of spermatocytes tested. Strains of TA97、TA98、TA100、TA102 were employed in Ames test. The exposure concentraton is 5000、1000、200、40ug/plate. Results:No significant difference were found in chromosome aberration and micronucleus rate between control and experimental groups. Ames test(in presence or absence of S9) showed no two fold increase in the spontaneous reversion colonies.There was no dose-dependence in the number of revertant colonies either.The result of Ames test is negative.Conclusion:The results suggested that Fenoxanil had no mutagenic activity.更多还原