【作者】 屠幼英； 程龙； 杨子银； 徐懿；

【Author】 TU Youying 1,CHENG Long 2,YANG Ziyin 1,Xu Yi 1 (1 Department of Tea Science,Zhejiang University,Hangzhou 310029,China;2 Wuxi Higher Health Vocational Technology School,Wuxi 210024,Jiangsu,China)

【机构】 浙江大学茶学系； 江苏省联合职业技术学院无锡卫生分院；

【摘要】 通过液质联用分析从茶花乙酸乙酯层中鉴定出4类化合物:a)儿茶素类:儿茶素、表儿茶素、没食子儿茶素、表没食子儿茶素、儿茶素没食子酸酯、表儿茶素没食子酸酯、没食子儿茶素没食子酸酯、表没食子儿茶素没食子酸酯;b)儿茶素衍生物:儿茶素糖苷物与儿茶素二聚体;c)黄酮苷类:山奈酚、毛地黄黄酮与槲皮素单糖苷及双糖苷类物质。经HPLC分离与DPPH测定,确定表没食子儿茶素没食子酸酯与表儿茶素没食子酸酯为茶花中主要抗氧化活性成分,在茶花抗氧化活性中起到主要的作用。更多还原

【Abstract】 High performance liquid chromatography (HPLC) combined with diode array (DAD) and electrospray (ESI)-ion trap-MS detection was used to separate and identify the compounds present in EEA.Eight catechins,namely,catechin,epicatechin (EC),gallocatechin,epigallocatechin,gallocatechin gallate,epigallocatechin gallate (EGCG),catechin gallate and epicatechin gallate (ECG),catechins derivatives,namely,EC-3’-dirhamnoside and catechins dimer,and flavonol glycosides,namely,quercetin,luteolin,kaempferide and kaempferol mono-,diglycosides (glucoside and rhamnoside) were identified.In addition,it is found that EGCG and ECG are the major active components responsible for the antioxidant activity of tea flowers by HPLC separation and DPPH assay.更多还原