Environment-differential association analysis of antibiosis to common cutworm in Chinese soybean germplasm population and optimization of cross design

【作者】 Hyunjee Kim； 邢光南； 贺建波； 赵团结； 杨守萍； 李艳； Reid G.Palmer； 盖钧镒；

【Author】 Hyunjee Kim;Reid G.Palmer;Department of Agronomy,Iowa State University,Ames,Iowa 50011,USA;

【机构】 南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室； Department of Agronomy,Iowa State University,Ames,Iowa 50011,USA；

【摘要】 Common cutworm(CCW)is one of the serious leaf-feeding pests of soybean,and causes significant yield losses in China.A sample of the Chinese germplasm population composed of 526soybean accessions was studied through association analysis using 233 polymorphic markers to detect QTL and corresponding alleles related to antibiosis to CCW with larva weight(LW)as indicator.A two-stage procedure with multi-loci genetic model at the second stage was used in association mapping,which was conducted for individual years due to very significant Accession x Year interaction in ANOVA.The results were environment-differential;26 and 43 QTL with their 217 and 421 alleles were detected in 2009 and 2011,respectively.Among those,ten loci on Chromosome 2,8,12,14,16 and 17 with total 124 alleles were common between years.Accordingly,the detected QTL-allele matrices of the population in individual years were established,which showed more allele effect variation in 2011 than in 2009.The breeding potential of possible combinations among the 526 accessions was estimated and the 10 top crosses common between the years were identified according to their predicted values.The best crosses were mainly from resistant by resistant accessions and transgressive progenies with LW much lower than the best accession(0~30 mg vs.149 mg)could be obtained.This study implies that the association analysis,with suitable markers and improved procedures,can detect many QTL/multi-alleles for a germplasm population and provide full genetic information in matrix for optimized cross design and marker-assisted progeny selection.更多还原

【Abstract】 Common cutworm(CCW) is one of the serious leaf-feeding pests of soybean,and causes significant yield losses in China.A sample of the Chinese germplasm population composed of 526 soybean accessions was studied through association analysis using 233 polymorphic markers to detect QTL and corresponding alleles related to antibiosis to CCW with larva weight(LW) as indicator.A two-stage procedure with multi-loci genetic model at the second stage was used in association mapping,which was conducted for individual years due to very significant Accession x Year interaction in ANOVA.The results were environment-differential;26 and 43 QTL with their 217 and 421 alleles were detected in 2009 and 2011,respectively.Among those,ten loci on Chromosome 2,8,12,14,16 and 17 with total 124 alleles were common between years.Accordingly,the detected QTL-allele matrices of the population in individual years were established,which showed more allele effect variation in 2011 than in 2009.The breeding potential of possible combinations among the 526 accessions was estimated and the 10 top crosses common between the years were identified according to their predicted values.The best crosses were mainly from resistant by resistant accessions and transgressive progenies with LW much lower than the best accession(0~30 mg vs.149 mg) could be obtained.This study implies that the association analysis,with suitable markers and improved procedures,can detect many QTL/multi-alleles for a germplasm population and provide full genetic information in matrix for optimized cross design and marker-assisted progeny selection.更多还原