【作者】 张海龙； 田兆丰； 董志； 张晏如； 张飞云；

【Author】 Hai long Zhang~1 Zhao feng Tian~2 Zhi Dong~1 Yan ru Zhang~3 Fei yun Zhang~1 (1 College of Life sciences,1 Capital Normal University,Beijing 100048) (2 Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097) (3 Beijing City University,Beijing 100083)

【机构】 首都师范大学生命科学学院； 北京市农林科学院植物保护环境保护研究所； 北京城市学院；

【摘要】 根据已报道的多花黑麦草斑驳病毒(RMotV)外壳蛋白(CP)基因的核苷酸序列合成引物,用RT-PCR的方法获得外壳蛋白基因后,再将其克隆至pGEX-4T-1中,SDS-PAGE和斑点杂交(Dot-blotting)表明:经1PTG诱导,CP基因在火肠杆菌BL21(DE3)plys中获得了高效正确的表达,并且表达产物以不溶性包涵体的形式存在。更多还原

【Abstract】 Based on published nucleotide sequence,coat protein gene of RMotV(Ryegrass Mottle Virus,RMotV) was synthesized by reverse transcription polymerase chain reaction (RT-PCR) and constructed into expression vector pGEX-4T-1.The result of SDS-PAGE showed that successful expression of a 50kD fusion protein was achieved by IPTG induction,and furthermore it was indicated by Dot-blotting that the fusion protein was expressed in the form of insoluble inclusion bodies更多还原