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断奶及日龄对仔猪骨髓组织抗菌肽PR-39基因表达的影响
Effect of Age and Weaning on the PR-39 Gene Expression in Femur Marrow of Piglets
【Author】 ZHAO Yu-Rong,ZHOU Can,FAN Zhi-Yong,HE Jian-Hua~* (College of Animal Science and Technology,HNAU,Changsha 410128,China)
【机构】 湖南农业大学动物科技学院；
【摘要】 分别于出生当天、3、14、23、28日龄随机屠宰杜长大公猪4头,采集骨股骨髓组织。用RT-PCR方法,以18S rRNA作内标,定量分析不同日龄仔猪骨髓组织抗菌肽PR-39 mRNA表达的差异及断奶对其表达的影响。结果表明:不同日龄仔猪骨髓组织PR-39 mRNA相对丰度有明显的差异(P<0.05)。出生时较低,吮吸初乳2天后显著增加(P<0.05),14日龄时较3日龄显著下降(P<0.05),随后随日龄的增加,其表达量呈上升趋势;断奶导致其显著下降(P<0.05)。
【Abstract】 In the present investigation an optimized semi-quantitative RT-PCR was applied to detect the PR-39 gene expression difference in femur marrow of piglets at different ages and weaning by using 18S rRNA as inner marker.Samples were taken at birth,3,14,23,28 days of age respectively.The results showed that age affected PR-39 mRNA levels in femur marrow significantly(P<0.05),the expression of PR-39 mRNA was the lowest at birth,then increased remarkably at 3 days of age,but the expression at 14 d-old was lower than that of 3 days(P<0.05),and it was increased thereafter.Weaning affected PR-39 mRNA levels significantly also(P<0.05).Weaning decreased the PR-39 mRNA levels at the same day.
【Key words】 Semi-quantitative RT-PCR； Piglets； weaning； Antibacterial peptide； PR-39； Gene expression；
- 【会议录名称】 格莱姆抗菌肽——抗菌肽开发与应用技术研讨会论文集
- 【会议名称】格莱姆抗菌肽——抗菌肽开发与应用技术研讨会
- 【会议时间】2009-12-14
- 【会议地点】中国广东深圳
- 【分类号】S828.5
- 【主办单位】中国农业产业经济发展协会