【作者】 潘华奇； 张淼； 刘丽； 王楠； 胡江春； 窦德强； 王书锦；

【Author】 PAN Hua-qi~1,ZHANG Miao~2,LIU Li~1,WANG Nan~1,HU Jiang-chun~(1*), DOU De-qiang~2,WANG Shu-jin~1 (1 Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;2 Liaoning University of TCM,Shenyang 110032,China)

【机构】 中国科学院沈阳应用生态研究所； 辽宁中医药大学；

【摘要】 从全国30个地区采集牛蒡根际土壤样品,进行了根际土壤真菌数量和群落的生态学研究,发现镰刀菌为牛蒡根际的最优势类群;通过测定镰刀菌发酵液对牛蒡幼苗和牛蒡子萌发的影响,发现镰刀菌对牛蒡有明显的毒性作用,对其中毒性最强的两株进行了形态学和分子生物学的鉴定,被鉴定为Fusarium solani。更多还原

【Abstract】 The thirty Arctium lappa L.rhizosphere soils were randomly collected from different areas in China. Fungal community and its dominant population of Arctium lappa L.rhizosphere were studied.Result shows that the dominant populations are Fusarium and Trichotderm.These Fusarium strains which are almost deleterious to the growth of Arctium lappa L.was found by treating seedlings and seeds of Arctium lappa L. with Fusarium strains fermentation broth.The most toxic Fusarium strains F130 and F131 have been identified using the method of morphology and phylogenetic analysis of the 5.8S gene and flanking internal transcribed spacer(ITS1 and ITS2)regions of nrDNA.The results indicated that strains F130 and F131 belonged to Fusarium solani.更多还原