【作者】 谢小保； 李文茹； 袁英姿； 欧阳友生； 陈仪本；

【Author】 Xie Xiao-bao~(1,2) Li Wen-ru~(1,2) Yuan Yingzi Zeng Hai-yan~(1,2) Ou-yang You-sheng~(1,2) Chen Yi-ben~(1,2*) Guangdong Institute of Microbiology Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application

【机构】 广东省微生物研究所； 广东省菌种保藏与应用重点实验室；

【摘要】 以金黄色葡萄球菌为研究对象,对纳米银的抑菌效果进行了研究,并对其抑菌机制做了初步探讨。纳米银对金黄色葡萄球菌的抑制生长曲线的结果表明,5.0μg/ml的纳米银能够完全抑制金黄色葡萄球菌细胞生长,纳米银使金黄色葡萄球菌的延滞期加长,并且纳米银浓度越高,延滞期越长。采用透射电镜观察了经纳米银粒子处理过的金黄色葡萄球菌细胞形态变化过程,结果显示纳米银粒子先在细胞壁上产生小的孔洞,通过这些孔洞进入周质空间,导致细胞膜成分渗漏和破坏细胞膜,进而进入细胞内部。进入细胞内部的纳米银粒子使DNA浓缩呈紧张态,并与破损细菌的细胞质结合积聚,最后引起胞内物质流失。另外,纳米银对金黄色葡萄球菌总DNA影响的分析表明,纳米银能够使金黄色葡萄球菌总DNA样品降解的程度增大。更多还原

【Abstract】 In this paper,the antimicrobial effect of the nanosilver on Staphylococcus aureus and the antimicrobial mechanism was studied elementarily.Experimental results indicated that the silver nanoparticles of 5.0μg/ml could inhibit completely the growth in MH medium.The growth curves showed that silver nanoparticles prolonged the lag phase of Staphylococcus aureus,and the higher of the concentration of silver nanoparticles,the longer of the lag phase of Staphylococcus aureus. Transmission electron microscopy(TEM) was used to evaluate the cell morphology of both the native and the treated Staphylococcus aureus.The observation with TEM suggested that silver nanoparticles lead to the formation of"pits"in cell wall of the bacteria,and silver nanoparticles could enter into periplasm through the pits and destroyed the cell membrane.Then the silver nanoparticles could enter into the bacterial cell,which not only condensed DNA,but also combined and coagulated with the cytoplasm of damaged bacteria.Finally,silver nanoparticles resulted in the leakage of cytoplasmic component.Moreover,the analysis of agar gel electrophoresis demonstrated that silver nanoparticles could increase the decomposability of genome DNA.更多还原