【作者】 王晨旭；

【导师】 马凤英；

【作者基本信息】 郑州大学 ， 测试计量技术及仪器， 2023， 硕士

【摘要】 电化学检测方法作为一种常见的定量定性分析方法,具有测定简单,灵敏度高,响应速度快等优点,广泛应用于材料、化工、生物、医疗等领域。但现有的电化学分析设备体积庞大笨重、价格昂贵,并且需要专业的人员在实验室内操作。这些因素极大地限制了其在面向非专业群体用户即时检测（Point-of-care Test,POCT）的应用场合。分析仪器的微型化对于实现方便的POCT现场和实时检测的需求迫在眉睫。本文拟研发一款基于ADu CM355微控制器的微电化学传感设备,使用业界领先的集成硬件,利用专业的电子以及软件专业知识将其激活,并设计一种共价有机骨架(COF_DATA-TP)材料修饰的丝网印刷电极（SPE）作为便携式电极传感器与硬件集成,基于不同方法采用该设备进行电化学测量,最后通过智能手机上的图形用户界面（Graphical User Interface,GUI）直观地显示测试图形和结果。主要内容包括如下四部分:首先是硬件方面,设计电路原理图并绘制PCB板,并对其调试以确定设备的主要性能指标。最终的硬件尺寸只有48mm×35mm,满足微型和便携的需求。其次是软件方面,包括硬件上的嵌入式底层程序、Android设备上的APP上位机软件以及通过USB实现硬件和上位机设备之间的供电和通信几部分。通过对三种电化学方法的测试,验证了能够通过APP显示检测结果,且结果准确可靠,满足实时检测的需求。然后设计并合成一种共价有机骨架(COF_DATA-TP)材料,用于修饰SPE,构建电化学传感器电极芯片,以提高电极表面的吸附能力从而提高设备的检测灵敏度,并且能同时检测重金属离子(Hg²⁺、Cu²⁺、Pb²⁺、Cd²⁺)。将COF_DATA-TP/SPE集成到硬件电路上,验证了构建微电化学设备思想的可行性。最后利用COF_DATA-TP/SPE构建的电化学传感器对Hg²⁺、Cu²⁺、Pb²⁺、Cd²⁺重金属离子进行检测,并与CHI760设备的检测结果对比,二者峰电位表现出显著的一致性,峰电流虽有轻微偏差但在一定浓度范围内仍与浓度呈现良好的线性关系。证明该微电化学传感设备在对重金属离子的实时在线检测方面具有良好的性能。更多还原

【Abstract】 As a common quantitative and qualitative analysis method,electrochemical detection method has the advantages of simple determination,high sensitivity,fast response,etc.,and it is widely used in materials,chemical,biological,medical and other fields.However,the existing electrochemical analysis equipment is bulky,expensive,and requires professional personnel to operate in the laboratory.These factors greatly limit its application in Point of Care Test for non-professional users.The miniaturization of analytical instruments is urgently needed to achieve convenient on-site and real-time POCT detection.This article intends to develop a micro electrochemical sensing device based on an ADu CM355 microcontroller.We use industry-leading integrated hardware,utilizes specialized electronic and software expertise to activate it.Then a covalent organic skeleton（COFDATA-TP）material modified screen printed electrode（SPE）is designed as a portable electrode sensor integrated with hardware.We use this device for electrochemical measurements based on different methods,and finally display the test graphics and results visually through the Graphical User Interface（GUI）on the smartphone.The main content includes the following four parts:First,in terms of hardware,design a circuit schematic diagram and draw a PCB board,and debug it to determine the main performance indicators of the device.The final hardware size is only 48 mm×35mm,meeting the needs of micro and portable.Second,in terms of software,it includes embedded underlying programs on hardware,APP host computer software on Android devices,and power supply and communication between hardware and host computer devices through USB.The testing of three electrochemical methods has proven that the device can display detection results through the APP,and the results are accurate and reliable.This meets the demand for real-time detection.Then,design and synthesis of a covalent organic skeleton(COF_DATA-TP)material for modifying SPEs and constructing electrochemical sensor electrode chips to improve the adsorption ability of the electrode surface,thereby improving the detection sensitivity of the device,and being able to simultaneously detect heavy metal ions(Hg²⁺,Cu²⁺,Pb²⁺,Cd²⁺).Integrating COF_DATA-TP/SPE into the hardware circuit verifies the feasibility of the idea of constructing a micro electrochemical device.Finally,an electrochemical sensor constructed using COF_DATA-TP/SPE was used to detect heavy metal ions such as Hg²⁺,Cu²⁺,Pb²⁺and Cd²⁺.Compared with the detection results of the CHI760 device,the peak potentials of the two showed significant consistency.Although the peak current had a slight deviation,it still showed a good linear relationship with the concentration within a certain concentration range.It has been proved that the micro electrochemical sensing device has good performance in real-time online detection of heavy metal ions.更多还原