【作者】 何琦；

【导师】 熊晶；

【作者基本信息】 南昌大学 ， 临床医学硕士（专业学位）， 2023， 硕士

【摘要】 目的:探讨血清中S100β蛋白、C反应蛋白水平和认知功能障碍之间的关系。材料和方法:本研究选取南昌大学第一附属医院就诊患者,其中52例认知障碍组(其中阿尔茨海默病AD组36例,轻度认知障碍MCI组16例),健康对照者16例(HC组),对所有纳入的受试者进行血清S100β、血清C反应蛋白检测以及神经心理测试、日常生活活动能力评估量表,分析不同认知组组间S100β蛋白和CRP水平的差异,运用相关性分析血清S100β蛋白、CRP水平与神经心理测试结果、日常能力评分、营养评分之间的关系,血清S100β蛋白、CRP在阿尔茨海默症的诊断价值预测用ROC曲线,ROC曲线的曲线下面积(AUC)比较诊断的准确性。结果:(1)MCI组、AD组的日常生活能力以及营养状况较HC组明显降低(p<0.001)。(2)AD组MMSE评分显著低于HC组(p<0.001)和MCI组(p<0.001)。AD组血清中S100β蛋白水平高于HC组(p=0.001)。三组间血清CRP水平差异无统计学差异(p>0.05)。(3)Spearman相关性分析显示血清S100β蛋白水平与MMSE评分呈负相关(r=-0.327,p<0.05)。(4)纳入S100β蛋白、CRP、学历、年龄、白蛋白、肌酐、BI指数、同型半胱氨酸进行多因素线性回归分析,结果发现血清S100β蛋白水平(p=0.041)、学历(p=0.026)、年龄(p=0.008)对认知功能存在影响。(5)进行ROC曲线分析,结果显示S100β蛋白水平的AUC=0.756(95%CI0.637–0.875)。结论:血清S100β蛋白水平与认知功能中存在负相关,血清CRP水平与认知功能评估无相关性。血清S100β水平可作为辅助阿尔茨海默病(AD)诊断的生物指标之一。更多还原

【Abstract】 Objective:Exploring the relationship between serum levels of S100β protein,C-reactive protein,and cognitive impairment.Methods:This study selected patients from the First Affiliated Hospital of Nanchang University,including 52 cases of cognitive impairment(36 cases of Alzheimer’s disease,16 cases of mild cognitive impairment)and 16 cases of healthy controls(HC group).All participants underwent neuropsychological testing and serum S100β and CRP detection.The serum concentrations of S100β protein and CRP were dissimilarly compared between different groups,and the correlation between serum S100β protein and CRP levels and daily living activity assessment scores and MMSE scores was analyzed using correlation analysis.The diagnostic value of serum S100βprotein and CRP in Alzheimer’s disease was predicted using ROC curves,and the diagnostic accuracy was compared using the area under the curve(AUC).Results:(1)The MCI and AD groups had a significantly lower ability to perform daily living as well as nutritional status compared to the HC group(p < 0.001).(2)MMSE scores in the AD group were significantly lower than those in the HC group(p < 0.001)and MCI group(p <0.001).Serum S100β protein levels in the AD group were higher than those in the HC group(p = 0.001).There was no statistical difference in serum CRP levels between the three groups(p < 0.05).(3)Spearman correlation analysis showed a negative correlation between serum S100β protein levels and MMSE scores(r=-0.327,p<0.05).(4)S100β protein,CRP,education,age,albumin,creatinine,BI index,and homocysteine were included in a multifactorial linear regression analysis,which revealed an effect of serum S100β protein level(p = 0.041),education(p = 0.026),and age(p = 0.008)on cognitive function.(5)ROC curve analysis was performed,and the results showed AUC=0.756(95% CI 0.637-0.875)for S100β protein level.Conclusions:Serum S100β protein level is negatively correlated with cognitive function,while serum CRP level is not related to cognitive function evaluation.Serum S100βlevels can be used as biomarkers to aid in the diagnosis of Alzheimer’s disease.更多还原