【作者】 刘颖；

【导师】 徐章润；

【作者基本信息】 东北大学 ， 分析化学， 2020， 硕士

【摘要】 生物微环境的酸度在人体中起着重要的作用,微环境的酸度不仅影响肿瘤的发生、恶性转化、转移和血管生成,同时也影响癌细胞对各种治疗的反应。对肿瘤微环境的酸度监测具有重要的意义。本文基于聚苯胺在不同pH条件下的质子化和去质子化导致拉曼信号强度和颜色的变化,提出了一种基于聚乙二醇二丙烯酸酯微粒(PEGDA)包封金纳米棒@聚苯胺(AuNRs@PANI)的比色-表面增强拉曼散射(Surface-enhanced Raman scattering,SERS)双模式pH传感方法。通过PANI包裹SERS增强基底AuNRs从而增强PANI的SERS信号。通过PEGDA包封AuNRs@PANI增强了传感器的稳定性和灵敏度。利用双模式传感器对细胞培养期间的培养基pH变化进行双模式监测。第一章,介绍了检测生物微环境pH值的意义和主要的检测方法;概述了金-聚苯胺纳米复合材料在SERS传感中的应用;其次介绍了凝胶微粒制备方法与应用;最后提出了本工作的研究意义及设计思想。第二章,首先制备AuNRs@PANI纳米粒子,之后利用微流控芯片制备了AuNRs@PANI/PEGDA凝胶微粒。对AuNRs@PANI纳米粒子进行了拉曼光谱、透射电子显微镜、吸收光谱表征;对AuNRs@PANI/PEGDA微粒进行了光学成像和拉曼光谱表征。解释了 AuNRs@PANI/PEGDA凝胶微粒在不同pH条件下SERS信号和颜色变化的原理,并利用其在不同pH条件下SERS信号和颜色变化,实现了细胞培养环境pH监测。该方法中,SERS监测在pH 5.4-8.0之间具有良好的线性,拟合曲线为y=-5707.46+1164.96x(R2=0.995);比色监测在pH5.5-8.0之间具有良好的裸眼可见的颜色变化,准确度为0.5个pH单位。第三章,对本文基于AuNRs@PANI/PEGDA凝胶微粒的比色-SERS双模式pH监测方法进行了总结,并对其应用前景进行了展望。更多还原

【Abstract】 The acidity of biological microenvironments plays an important role in human body.The acidity of the microenvironments not only affects the occurrence,malignant transformation,metastasis and angiogenesis of tumors,but also affects the response of cancer cells to various treatments.Therefore,it is of great significance to monitor the acidity of tumor microenvironments.In this paper,based on the change of Raman signal intensity and color caused by protonation and deprotonation of polyaniline at different pHs,a colorimetry and surface-enhanced Raman scattering(SERS)dual-mode pH sensing method based on gold nanorod@polyaniline(AuNRs@PANI)-embedded polyethylene glycol diacrylate microparticles(PEGDA)was proposed.SERS signal was enhanced by coating AuNRs with PANI.The stability and sensitivity of the sensor was enhanced by encapsulating AuNRs@PANI with PEGDA.The pH change of culture medium during cell culture was monitored by using the dual-mode pH sensor.In chapter 1,firstly,the significance of detecting biological microenvironment pH and the main detection methods of pH were introduced.The applications of gold-polyaniline nanocomposites in SERS sensing were reviewed.Secondly,the preparation methods and applications of gel microparticles were introduced.Finally,the significance and design ideas of this work were put forward.In chapter 2,AuNRs@PANI nanoparticles were prepared firstly.Then AuNRs@PANI/PEGDA gel microparticles were prepared on a microfluidic chip.AuNRs@PANI nanoparticles were characterized by Raman spectroscopy,absorption spectroscopy and transmission electron microscopy.AuNRs@PANI/PEGDA microparticles were characterized by optical imaging and Raman spectroscopy.SERS signal and color change of AuNRs@PANI/PEGDA gel microparticles under different pHs were used to realize pH monitoring of cell culture environment.The SERS detection method has good linearity within pH 5.4-8.0.The fitting curve is y=-5707.46+1164.96x(R2=0.995).AuNRs@PANI/PEGDA also exhibited vivid color change within the physiological pH range 5.5-8.0,and was used to visualize pH change with a precision of 0.5 pH unit.In chapter 3,the colorimetry and SERS dual-mode pH detection method based on AuNRs@PANI/PEGDA gel microparticles was summarized,and its application was prospected.更多还原