【作者】 张颖；

【导师】 吴剑荣；

【作者基本信息】 江南大学 ， 制糖工程， 2022， 硕士

【摘要】 结冷胶与聚赖氨酸通过湿法纺丝技术可制备出性质可调的复合纤维。目前结冷胶-聚赖氨酸复合纤维（Gellan-Polylysine,GPL）应用于医用纺织类材料的研究还较少,并且多糖类纤维的功能较为单一,这些都限制了GPL纤维在不同极端条件下的使用潜力。本研究旨在制备一种含有营养物质且具有抗菌、促伤口愈合等功能的GPL纤维,并对其理化性质和伤口敷料潜力进行初步探究。主要研究结果如下:（1）对纺丝条件及纺丝原液组分进行优化,建立含有营养物质的湿法纺丝体系。当以5%（w/v）聚赖氨酸溶液为固定液,以1%（w/v）结冷胶（其中低酰基与高酰基结冷胶比例为5:1）、1.0%（w/v）葡萄糖、0.5%（w/v）大豆肽和2.0%（w/v）鱼胶原蛋白肽的混合物作为纺丝原液（加入顺序为鱼胶原蛋白肽→大豆肽→葡萄糖）;纺丝原液与固定液恒温75℃,推进器泵出速度为0.5 m L·min^-1,纺丝机转速为20 r·min^-1时,制备出的纤维质量较高,在该条件下纺丝原液粘度为35.03±0.04 cp,纤维断裂强度可达到0.47±0.03 c N·dtex^-1。（2）对含有营养物质的GPL纤维的理化性质进行表征。GPL纤维表面平滑,孔隙均匀;吸水膨胀率在100%以上;GPL纤维在经过胃液消化2 h后葡萄糖释放率为98.24±5.80%;经胃肠液消化时肽类物质逐渐分解为小分子肽段;纤维中As、Pb、Hg含量在食用标准范围内。（3）对包埋药物脂质体的制备条件进行优化,建立含人参皂苷、百里香酚以及维生素K1的脂质体包埋体系。当卵磷脂:胆固醇=4:1、卵磷脂:吐温-80=2.5:1.8、卵磷脂:百里香酚=100:7、卵磷脂:人参皂苷=9:1、卵磷脂+胆固醇:维生素K1=5:1、油相:水相=1:3时,制备的脂质体质量较高,形态饱满圆滑,大小均一,分布均匀,粒径为183.7±15.4nm,PDI为0.233±0.020。人参皂苷的包埋率为91.27±2.57%,百里香酚的包埋率为37.14±2.53%,维生素K1的包埋率为29.24±1.50%。（4）对含有脂质体的多功能GPL纤维应用于伤口敷料的潜力进行初步探究。多功能GPL纤维对大肠杆菌以及金黄色葡萄球菌的生长均有一定的抑制作用;纤维菌落总数为0 cfu·g^-1;纤维溶血率为4.10±0.66%;使用不同浓度和不同浸提时间的纤维浸提液处理L-929细胞后,细胞存活率均大于等于90%;大鼠伤口愈合实验显示,多功能GPL纤维处理患处7 d后,大鼠伤口基本愈合且伤口周围已生长出新的毛囊以及毛发,伤口愈合率达到95±5%;伤口组织切片观察显示出现肉芽组织,造模处上皮层基本愈合。更多还原

【Abstract】 Gellan gum and polylysine can be used in wet spinning to produce fibers with adjustable properties.At present,there is few researchs on the application of Gellan-Polylysine fiber（GPL）in medical textile materials,and the function of polysaccharide fiber is still relatively simple,these limit the potential use of GPL fibers under different extreme conditions.The purpose of this study was to prepare a nutrient-containing GPL fiber with antibacterial and wound healing properties,and to explore its physicochemical properties and wound dressing potential.The main findings were as follows:（1）To optimized the spinning conditions and the composition of spinning solution,we established a wet-spinning system containing nutrients.When the fixing solution was poly-L-lysine（5%w/v）,the spinning solution was composed of gellan（1%w/v）that the ratio of high acyl gellan to low acyl gellan was 1:5,glucose（1.0%w/v）,soybean peptide（0.5%w/v）and fish collagen peptide（2.0%w/v）,the sequence of addition was collagen peptide,soybean peptide and glucose,the comprehensive properties of the fiber were the best when the spinning solution and the fixing solution holded at 75℃,the speed of automatic propeller was 0.5 m L·min^-1 and the speed of spinning stock was 20 r·min^-1.The viscosity of spinning solution was 35.03±0.04 cp,the breaking strength of GPL fiber was 0.47±0.03 c N·dtex^-1.（2）The physical and chemical properties of GPL fiber containing nutrients were characterized.The surface of the fiber was smooth and the pore was even.The swelling rate of the fiber was more than 100%.The glucose release rate of GPL fiber was 98.24±5.80%after 2 hours of gastric digestion.The contents of As,Pb and Hg in the fiber were within the range of edible standard.（3）The preparation conditions of liposomes containing drugs were optimized,and an entrapment system containing ginsenoside,thymol and Vitamin K1 was established.When the ratio of lecithin to cholesterol was 4:1,the ratio of lecithin to Tween-80 was 2.5:1.8,the ratio of lecithin to thymol was 100:7,the ratio of lecithin to ginsenoside was 9:1,the ratio of lecithin and cholesterol to vitamin K1 was 5:1,and the ratio of oil phase to water phase was1:3,the morphology of the liposomes were smooth,similar in size and uniform in distribution.The particle size was 183.7±15.4 nm and PDI was 0.233±0.020.The encapsulation efficiency of ginsenoside,thymol and vitamin K1 were 91.27±2.57%,37.18±2.53%and 29.24±1.50%respectively.（4）The potential application of multifunctional GPL fiber containing liposome in wound dressings was investigated.The multifunctional GPL fiber inhibited the growth of Escherichia coli and Staphylococcus aureus,and the total number of bacterial colonies was 0 cfu·g^-1.The hemolysis rate of GPL fiber was 4.10±0.66%,the cell survival was more than 90%under the treatment of different concentration and time of extraction.After 7 days of fiber treatment,the wound was basically healed and new hair follicles and hair grew around the wound.The wound healing rate was 95±5%.Microscopic examination of the wound tissue showed the appearance of granulation tissue,and the epithelial layer of the model was basically healed.更多还原