【作者】 孙俊杰；

【导师】 金百冶；

【作者基本信息】 浙江大学 ， 外科学（泌尿）（专业学位）， 2021， 硕士

【摘要】 研究背景及目的:肿瘤微环境(TME)在肾透明细胞癌(KIRC)的发生和发展中起着至关重要的作用。然而,对肿瘤微环境免疫浸润的认识尚不清楚。本研究旨在探讨肿瘤微环境(TME)与肾透明细胞癌临床特征及预后的关系。研究方法:在本研究中,我们应用ESTIMATE和CIBERSORT计算方法,从肿瘤基因组图谱(TCGA)数据库中计算出肾透明细胞癌(KIRC)中肿瘤浸润免疫细胞(TIC)的比例和免疫和基质成分的数量,并确定可能起核心作用的免疫细胞和基因的种类,然后在GEO数据库中进行验证。研究结果:单因素COX与PPI交叉分析确定血清淀粉样蛋白A1(SAA1)为预测因子。SAA1的表达与OS呈显著负相关,与临床TMN分期呈正相关。SAA1高表达组基因主要富集于免疫相关活性。静止状态的肥大细胞比例与SAA1表达呈负相关,提示SAA1可能参与肿瘤微环境免疫状态的维持。结论:SAA1可能是肾透明细胞癌患者预后判断的新指标,并可能通过肥大细胞静息在肿瘤微环境中发挥重要作用。更多还原

【Abstract】 Background:Tumor microenvironment(TME)plays a crucial role in the initiation and development of clear cell renal cell carcinoma(cc RCC).However,the understanding of immune infiltration in TME is still unknown.Our study aims to investigate the association between TME and the clinical features as well as the prognosis of cc RCC.Methods:In the present study,we applied ESTIMATE and CIBERSORT computational methods to calculate the proportion of tumor-infiltrating immune cell(TIC)and the amount of immune and stromal fraction in kidney renal clear cell carcinoma(KIRC)form The Cancer Genome Atlas(TCGA)database and identify the kind of immune cell and genes which may play the central role,then validated in GEO database.Results:Serum Amyloid A1(SAA1)was determined as a predictive factor by the intersection analysis of univariate COX and PPI analysis.Expression of SAA1 was significantly negatively correlated to OS and positively correlated to clinical TMN stage system.Genes in the high-expression SAA1 group were mainly enriched in immune-related activities.The proportion of mast cells resting was negatively correlated with SAA1 expression,indicating that SAA1 may involve in the maintenance of immune status for TME.Conclusions:SAA1 may be a novel maker for outlining the prognosis of KIRC patients and may play a vital role in TME by mast cell resting.更多还原