【作者】 吴琼；

【导师】 王驰； 杨西斌；

【作者基本信息】 上海大学 ， 测试计量技术及仪器， 2021， 硕士

【摘要】 激光散斑衬比成像(Laser speckle contrast imaging,LSCI)具有结构简单、时空分辨率高、快速以及实时全场成像等优势,在生物组织的血流监测方面具有很大的发展潜力。但受限于光学成像的穿透深度,基于该技术的血流成像局限于组织表层,当需要实时在体监测生物体深层组织或腔内组织血流分布及变化时,将LSCI与腹腔镜成像技术相结合是解决LSCI成像深度的有效途径。但在已有的研究中,缺乏对腹腔镜LSCI成像系统影响因素的综合性分析,其血流测量也往往局限于定性测量。因此,本文选择对腹腔镜LSCI定量血流测量方面展开研究,设计搭建了商用腹腔镜LSCI成像系统,通过仿体实验和动物实验相结合的方法,实现了深层组织的血流成像,本文的主要研究成果如下:(1)系统分析了影响LSCI定量测量的关键参数,校正了静态散射和相机噪声对散斑衬比度的影响,通过多曝光散斑成像(Multi-exposure speckle imaging,MESI)方法确定电场自相关函数的相关时间,从而实现腹腔镜LSCI血流的定量测量。(2)针对MESI方法存在的采样时间长的问题,通过空间散斑衬比度和时间散斑衬比度来校正静态散射的影响,在单曝光时间下实现腹腔镜LSCI血流的定量测量,解决了单曝光LSCI定量分析能力差的问题。(3)将商用腹腔镜与LSCI相结合,在兔子的耳背处实现了在体MESI血流定量监测;对兔子腹腔内大肠血管进行在体阻断再灌注实验,基于单曝光LSCI获得了大肠血流的实时变化。更多还原

【Abstract】 Laser speckle contrast imaging(LSCI)has the advantages of simple structure,high temporal and spatial resolution,fast speed,and real-time full-field imaging.It has great development potential in the blood flow monitoring of biological tissues.But it is limited by the penetration depth of optical imaging.Blood flow imaging based on this technology is limited to the surface of the tissue.When it is necessary to monitor the blood flow distribution and changes in deep tissues or intracavitary tissues in vivo in real time,the combination of LSCI and laparoscopic imaging technologies is an effective way to solve the LSCI imaging depth.However,in the previous research,it lacks a comprehensive analysis of the influencing factors of the laparoscopic LSCI imaging system.The blood flow measurement is often limited to qualitative measurement.Therefore,this article chooses to carry out research on laparoscopic LSCI quantitative blood flow measurement,and builds a commercial laparoscopic LSCI imaging system.Through a combination of phantom experiment and animal experiment,the blood flow imaging of deep tissues is realized.The research results are as follows:The system analyzes the key parameters that affect the LSCI quantitative measurement,corrects the effects of static scattering and camera noise on the speckle contrast,and determines the correlation of the electric field autocorrelation function through the multi-exposure speckle imaging(MESI)method to achieve quantitative measurement of laparoscopic LSCI blood flow.Aiming at the problem of long sampling time in MESI,the effect of static scattering is corrected by spatial speckle contrast and temporal speckle contrast,and the quantitative measurement of laparoscopic LSCI blood flow is achieved under a single exposure time,which solves the problem of poor quantitative analysis ability in LSCI.It combines the commercial laparoscopy with LSCI to achieve quantitative monitoring of MESI blood flow in the rabbit’s ears;it conducts vivo block reperfusion experiments on the large intestine blood vessels in the abdominal cavity of rabbits,based on single exposure LSCI to obtain real-time large intestinal blood flow changes.更多还原