【作者】 张敏；

【导师】 杨潇潇； 余茂耘；

【作者基本信息】 合肥工业大学 ， 制药工程（专业学位）， 2021， 硕士

【摘要】 脱发(alopecia)是皮肤科中一种较为常见的疾病,其发病率逐年上升且呈年轻化趋势发展,困扰着越来越多人的生活。烫伤(scald)是最有害和最复杂的物理伤害,烫伤常会造成组织损伤。虽然脱发和烫伤的药物有一定的作用,但治疗效果有限,并且还伴有一定的副作用和后遗症,因此研发新的安全有效治疗脱发和烫伤的药物成为了研究热点。霍山石斛是兰科种属,在我国被用作草药已有数百年的历史。霍山石斛含有多糖、黄酮类化合物和生物碱等有效成分,并且有研究表明霍山石斛对于糖尿病、炎症、肿瘤以及肝损伤等方面都有一定的治疗作用,但是对于脱发及烫伤作用的研究仍是匮乏的。通过构建脱发小鼠模型后给予小鼠背部涂抹给药,持续两周,探究霍山石斛对脱发的作用。实验结果显示霍山石斛可以促进脱发小鼠的毛发生长,改善脱发小鼠毛囊的形态结构,并且促进脱发小鼠的毛囊生长。机制上,发现霍山石斛可以促进抑制凋亡基因B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)的表达,同时调控自噬关键基因p62和微管相关蛋白1轻链3-?(microtubule-associated protein 1 light chain3-beta,LC3II)的表达来激活自噬,诱导毛发进入生长期,并且通过抑制转换生长因子?1(transforming growth factor?1,TGF-?1)的表达延长了脱发小鼠毛发的生长期从而促进脱发小鼠毛发的生长。此外,通过构建烫伤小鼠模型后给小鼠烫伤处涂抹给药,持续两周,探究霍山石斛对于烫伤的治疗作用。结果显示霍山石斛具有促进伤口愈合的作用,随后通过H&E以及Masson染色发现霍山石斛治疗组烫伤处的上皮再生以及胶原蛋白显著增加。机制上,发现霍山石斛可以促进?-平滑肌肌动蛋白(?-smooth muscle actin,?-SMA)的表达,并且通过调节炎症抑制凋亡促进烫伤小鼠烫伤处的伤口愈合。综上所述,本研究证明了霍山石斛可以促进脱发小鼠的生发作用,改善毛囊的形态结构。此外,霍山石斛还可以促进烫伤小鼠的伤口愈合。我们的研究为霍山石斛治疗脱发和伤口损伤提供了理论基础。更多还原

【Abstract】 Alopecia is a relatively common disease in dermatology,and its incidence is increasing year by year and showing a younger trend,which is plagued the lives of more and more people.Scald is the most harmful and complex physical injury,which often causes tissue damage.The drugs of alopecia and scald have a certain effect,but the therapeutic effects on alopecia and scars is limited.Therefore,the development of new and effective drugs is a research hotspot.Dendrobium huoshanense is a species of Orchidaceae and has been used as herbal medicine in our country for hundreds of years.Dendrobium huoshanense contains active ingredients such as polysaccharides,flavonoids and alkaloids.Many studies have shown that Dendrobium huoshanense has certain therapeutic effects on diabetes,inflammation,cancer,and liver damage.However,if Dendrobium huoshanense has therapeutic effects on alopecia and scald is still unknown.In this study,constructed the mouse model of alopecia,and treated with Dendrobium huoshanense for two weeks.The results showed that Dendrobium huoshanense can promote hair growth,and improve the morphology and structure of hair follicles in alopecia mice.Mechanically,found that Dendrobium huoshanense can inhibit apoptosis by promoting expression of anti-apoptotic gene B-cell lymphoma-2(Bcl-2),activate autophagy to induce hair to the anagen phase and prolong the anagen phase by regulating expression of autophagy-related genes p62 and microtubule-associated protein 1 light chain 3-beta(LC3II),and promote hair growth by inhibiting transforming growth factorβ1(TGF-?1)in alopecia mouse.In addition,used scalded mouse model and treated mice with Dendrobium huoshanense for two weeks.The results showed that Dendrobium huoshanense can promote wound healing.The results of H&E and Masson staining indicated that Dendrobium huoshanense can promote epithelial regeneration and collagen deposition in the scalded area.Furthermore,found that Dendrobium huoshanense can promote collagen regeneration by inducing expression of ?-smooth muscle actin(?-SMA),and improve wound healing by regulating inflammation and inhibiting apoptosis.In summary,this study demonstrates that Dendrobium huoshanense can promote hair growth,improve the morphology and structure of hair follicles in alopecia mice.In addition,Dendrobium huoshanense can also promote wound healing in scald mouse model.Our research provides a theoretical basis for the treatment of alopecia and scald by Dendrobium huoshanense.更多还原