【作者】 冯波；

【导师】 高峰；

【作者基本信息】 内蒙古农业大学 ， 农业硕士（专业学位）， 2021， 硕士

【摘要】 本试验旨在研究妊娠后期限饲对蒙古绵羊胎盘发育和胎盘子叶毛细血管生成及相关基因的影响。试验选取体况中等,2-3胎次健康的蒙古绵羊24只,经同期发情受孕。在妊娠90天时选择6只具有代表性的母羊进行屠宰,剩余母羊随机分配到三个营养水平处理组:(限制1组:0.18MJ ME·BW^-0.75·d^-1,n=6;限制2组:0.33MJ ME·BW^-0.75·d^-1,n=6;对照组:0.63MJ ME·BW^-0.75·d^-1,n=5),进行不同程度的限制饲养至妊娠130天,对母羊进行屠宰取样。结果表明:1、妊娠后期限饲影响了蒙古绵羊胎盘生长发育和胎盘子叶类型,妊娠后期限饲导致蒙古绵羊胎儿的重量极显著降低（p<0.01）,其中MU1组胎盘重量显著低于CG组（p<0.05）。A、B类型胎盘子叶的重量和数量都降低（p>0.05）,并且出现C、D类型的胎盘子叶。2、妊娠后期限饲改变了蒙古绵羊胎盘子叶血管数量,在不同组别中A、B类型胎盘子叶血管数量显著降低（p<0.05）,但在同组别中A、B、C、D型胎盘子叶的血管数量依次增多（p<0.05）。3、妊娠后期限饲影响了蒙古绵羊胎盘子叶血管调控因子以及营养转运因子的表达,低营养组中各类型胎盘子叶的血管发育调控因子VEGF（p<0.01）、ANGPT1（p<0.01）、PLGF（p>0.05）的基因表达呈现不同程度降低,但受体KDR和FLT基因表达差异不显著（p>0.05）。营养转运调控因子GLUT1和SNAT2基因表达有下降趋但差异不显著（p>0.05）。更多还原

【Abstract】 This study was to investigate effects of feed restriction during late pregnancy on placental development,placental cotyledon angiogenesis in Mongolia Ovine.Twenty four Mongolian sheep with moderate body condition and healthy 2-3 parity were selected in the experiment,and they were pregnant by estrus synchronization.Six representative ewes were slaughtered on the 90th day of gestation,and the remaining ewes were randomly assigned to three nutrition levels treatment groups(Restriction group 1:0.18MJ ME·BW^-0.75·d^-1,n=6;Restriction group 2:0.33MJ ME·BW^-0.75·d^-1,n=6;Control group:0.63MJ ME·BW^-0.75·d^-1,n=5).Different degrees of nutritional restriction were carried out.After 130 days of gestation,the ewes were slaughtered and sampled.The results show that:1.Feeding restriction in late pregnancy affected the placental growth and development and the type of placental cotyledons of Mongolian sheep.Feeding restriction in late pregnancy significantly reduced the fetal weight of Mongolian sheep（p<0.01）,and the placental weight of mu1 group was significantly lower than that of CG group（p<0.05）.A.The weight and number of type B placental cotyledons decreased p>0.05),and type C and D placental cotyledons appeared.2.Feeding restriction in late pregnancy changed the number of vessels in fetal cotyledons of Mongolian sheep.In different groups,the number of vessels in cotyledons of type A and B placentas decreased significantly（p<0.05）,but in the same group,the number of vessels in cotyledons of type A,B,C and D placentas increased in turn（p<0.05）.3.The gene expression of vascular development regulators VEGF（p<0.01）,ANGPT1（p<0.01）and Pl GF（p>0.05）in different types of placental cotyledons in the low nutrition group were decreased in different degrees,but the gene expression of receptor KDR and FLT were not significantly different（p>0.05）.The gene expression of GLUT1 and SNAT2 decreased,but the difference was not significant（p>0.05）.更多还原