【作者】 梁爽；

【导师】 李灿宇；

【作者基本信息】 郑州大学 ， 妇产科学（专业学位）， 2021， 硕士

【摘要】 目的:探究COX-2选择性抑制剂塞来昔布对子宫腺肌病的作用及其与环氧合酶-2(cyclooxygenase-2,COX-2),血管内皮生长因子(vascular endothelial growth factor,VEGF),神经生长因子(nerve growth factor,NGF),促肾上腺皮质激素释放激素(corticotropin-releasing hormone,CRH)因子表达的关系。方法:通过建立小鼠子宫腺肌病模型并使用塞来昔布治疗子宫腺肌病,将新生雌性小鼠随机分为对照组,干预组,实验组,每组6只,造模后饲养3个月,收集小鼠子宫组织,采用HE染色观察子宫内膜浸润深度。采用反转录-聚合酶链反应(RT-PCR)法和Western-blot检测各组小鼠环氧合酶-2(COX-2),血管内皮生长因子(VEGF),神经生长因子(NGF),促肾上腺皮质激素释放激素(CRH)mRNA和蛋白的表达。结果:(1)对照组、干预组、实验组小鼠12周龄子宫重量逐渐增加,两两比较(P=0.009、P=0.000、P=0.001),P<0.05差异具有统计学意义。(2)对照组、干预组、实验组子宫内膜浸润深度两两比较(P=0.072、P=0.000、P=0.002),P<0.05差异具有统计学意义(3)干预组、实验组COX-2、VEGF、NGF和CRH mRNA和蛋白的表达量均高于对照组,差异具有统计学意义(P<0.001),干预组COX-2、VEGF表达明显低于实验组(P<0.001),但NGF和CRH的表达无明显差异(P>0.05)。结论:(1)COX-2选择性抑制剂塞来昔布可减轻小鼠子宫腺肌病程度。(2)COX-2、VEGF、NGF、CRH可能参与子宫腺肌病的发生、发展。(3)塞来昔布有望成为治疗子宫腺肌病的一种潜在的有效药物。更多还原

【Abstract】 Objective:This study aimed to explore the effect of COX-2 selective inhibitor(celecoxib)on adenomyosis and its relationship with cyclooxygenase-2(COX-2),vascular endothelial growth factor(VEGF),nerve growth factor(NGF),corticotropin-releasing hormone(CRH).Methods:By establishing a mouse model of adenomyosis and using celecoxib to treat adenomyosis,newly born female mice were randomly divided into control group,intervention group,and experimental group,with 6 mice in each group.After the model was established,they were reared for 3 months and collected the uterine tissues.hematoxylin-eosin(H&E)staining was used to observe the depth of endometrial infiltration of mouse adenomyosis.RT-PCR(reverse transcription PCR)and Western blot analysis were used to detect the expression of Cyclooxygenase-2(COX-2),Vascular endothelial growth factor(VEGF),Nerve growth factor(NGF),Corticotropin-releasing hormone(CRH)mRNA and protein in mice before and after celecoxib treatment.Results:(1)the uterus weight of mice aged 12 weeks in control group,intervention group and experimental group gradually increased(P=0.009,P=0.000,P=0.001),and the difference was statistically significant(P<0.05).(2)The endometrial infiltration depth of control group,intervention group and experimental group was compared in pairs(P=0.072,P=0.000,P=0.002),and the difference was statistically significant(P<0.05).The mRNA and protein expressions of COX-2,VEGF,NGF and CRH in intervention group and experimental group were higher than those in control group(P < 0.001),while the expressions of COX-2 and VEGF in intervention group were significantly lower than those in experimental group(P< 0.001),but there was no significant difference in NGF and CRH(P> 0.05).Conclusion:(1)A selective COX-2 inhibitor,celecoxib can reduce the degree of adenomyosis in mice.(2)COX-2,VEGF,NGF and CRH may be involved in the occurrence and development of adenomyosis.(3)Celecoxib is expected to be a potentially effective drug for the treatment of adenomyosis.更多还原