【作者】 贾玲；

【导师】 齐斌；

【作者基本信息】 东北师范大学 ， 分析化学， 2021， 硕士

【摘要】 盐酸麻黄碱(EPh)是一种拟交感神经胺类药物,作为常规药物主要用于治疗支气管哮喘和低血压,有时也被用作食欲抑制剂。EPh在生物体内过量存在时表现出类似甲基苯丙胺(冰毒)的性质,可引起神经紧张、心跳加速、高血压和癫痫,已经被国际奥委会医学委员会列入禁药名单。同时,EPh也是制造冰毒的重要原料之一,被我国列入了第一类易制毒化学品名单。为了打击EPh的滥用和毒品犯罪,迫切需要有一种简单,行之有效的EPh分析检测方法。在本工作中,充分利用了分子印迹技术,电化学方法,FRET理论以及荧光分析法,发展了两种EPh的分析新方法。具体工作内容如下:1.基于分子印迹技术,利用溶胶-凝胶法合成了分子印迹聚合物(MIP),MIP与Nafion-MWCNTs结合形成的复合材料作为检测EPh的电化学传感器,并对该传感器的电化学行为进行了评价。评价结果表明,该传感器具有良好的选择性、稳定性、重现性和抗干扰性能。在最佳条件下,传感器对EPh检测的线性范围为0.18-75μM,检出限达到了0.072μM(S/N=3),超越了很多之前报道过的工作。同时,在实际样品分析中该传感器也显示出了良好的分析性能。本工作不仅为EPh的分析检测提供了一种新的方法,也可以作为一种通用的分析方法拓展到其他物质的分析领域。2.以MPA作CdTe的包覆剂,利用水相合成法制备了MPA-CdTe。以MPA-CdTe与罗丹明B的荧光淬灭体系作为基础,FRET原理为指导,制备了“on-off”型EPh荧光传感器。该传感器对EPh的检测具有很高的灵敏度,检出限可以达到0.037μM,分析能力高于很多已有的报道,对实际样品的检测也取得了令人满意的结果。这一研究为基于QDs与FRET原理的高灵敏荧光传感器的设计和构建开辟了一条新的途径。更多还原

【Abstract】 Ephedrine hydrochloride(EPh)is a sympathomimetic amine,which is mainly used as a conventional drug for the treatment of bronchial asthma and hypotension.Sometimes it is also used as an appetite suppressant.EPh may exhibits methamphetamine-like properties when present in excess in living organisms,including nervousness,rapid heartbeat,hypertension and epilepsy.So it was placed in the list of banned substances by the IOC Medical Committee.Furthermore,EPh also is an important ingredient in the manufacture of methamphetamine which make it included in the list of Class 1 easily controlled chemicals in China.In order to combat EPh abuse and drug crime,there is an urgent need for a simple and proven method for the analysis of EPh.In this work,two new methods for the analysis of EPh have been developed making full use of molecular imprinting technology,electrochemical methods,FRET(F?rster resonance energy transfer)theory and QDs.Details of the work are as follows:1.Based on molecular imprinting technology,a molecularly imprinted polymer(MIP)was synthesized by sol-gel method.The composite material formed by combining MIP with Nafion-MWCNTs was used as an EPh sensor and the analytical performance of the present sensor was evaluated.The evaluation results showed that the sensor has good selectivity,stability,reproducibility and interference resistance ablitity.Under optimal conditions,the linear range of the sensor for EPh detection was 0.18 μM-75 μM and the detection limit reached 0.072 μM(S/N = 3),surpassing much of the previously reported work.The sensor also showed good analytical performance in the analysis of real samples.The present work can not only provides a new method for the analytical detection of EPh,but also be extended as a general analytical method for the analysis of other substances.2.MPA-CdTe were prepared by aqueous phase synthesis using mercaptopropionic acid as the capping agent for CdTe QDs.The fluorescence quenching system of MPA-CdTe and rhodamine B was used as the basis and the "FRET principle" was used as a guide to prepare a "on-off" type EPh fluorescence sensor.The detection limit of EPh was 0.037 μM,which is higher than many reports.Satisfying results were obtained on the actual samples,and this study opens a new avenue for the design and construction of highly sensitive fluorescence sensors based on the QDs and FRET principle.更多还原