【作者】 张超；

【导师】 姜伟；

【作者基本信息】 电子科技大学 ， 生物化学与分子生物学， 2020， 硕士

【摘要】 研究目的:了解四川省地区HCV感染情况、基本流行性特征以及基因型分布情况。研究方法:通过回顾性研究2014年-2018年期间采集自四川省人民医院4465例就诊患者的血液标本,对患者血液样本的HCV-RNA的病毒载量进行分析。同时,分析基于PCR-荧光探针分型法检测的从2016年-2018年期间收集的467例患者血液标本的HCV基因分型的情况。研究结果:高精度HCV-RNA定量检测确认阳性1647例,阳性率为36.89%,(95%可信区间:35.47-38.31%),其中813位为男性,阳性率为49.36%;女性为834,阳性率为50.64%。在阳性患者中40<年岁≤60岁患者占55.74%,在2014年-2018年期间41至60岁年龄组的阳性率分别为50.00%,57.94%,55.97%,55.51%和54.08%。每年进行HCV-RNA定量检测的确认阳性率分别为49.28%,48.98%,39.83%,30.47%和31.36%。2016年-2018年的469例HCV-RNA阳性血清标本中,1b型有355例(76.02%),占主导地位,男性166例(46.76%)和女性189例(53.25%)。研究结论:2014年至2018年四川省人民医院HCV-RNA确诊阳性率呈现逐年下降的趋势,40<年龄≤60岁患者阳性率却呈现一个逐年上升的趋势。四川地域HCV风行的基因型呈现多基因型的散布特色,首要为1b型。了解HCV感染患者的基本情况以及常见的分型,对临床检测与治疗有重要价值。更多还原

【Abstract】 OBJECTIVE: To investigate the HCV infection,basic epidemic characteristics and genotype distribution in Sichuan Province.METHODS: The viral load of HCV-RNA in blood samples from 4465 patients in the Sichuan Provincial People’s Hospital was analyzed by retrospective study from 2014 to 2018.At the same time,the HCV genotyping of 467 patient blood samples collected from 2016 to 2018 based on PCR-fluorescent probe typing was analyzed.RESULTS: 1647 positive cases were confirmed by high precision HCV-RNA quantitative test,the positive rate was 36.89%(95% confidence interval: 35.47-38.31%),of which 813 were male and the positive rate was 49.36%;the female was 834,the positive rate was 50.64%.Among the positive patients,55.74% were 41-60 years old,and the positive rates in the 41-60 age group in the five years(2014-2018)were 50.00%,57.94%,55.97%,55.51% and 54.08%,respectively.From 2014 to 2018,the positive rates of HCV-RNA quantitative tests were 49.28%,48.98%,39.83%,30.47%and 31.36%,respectively.Of the 467 HCV-RNA positive serum specimens from 2016 to 2018,355 cases(76.02%)of type 1b were predominated,with 166 males(46.76%)and 189 females(53.25%).CONCLUSIONS: From 2014 to 2018,the positive rate of HCV-RNA detected in the Sichuan Provincial People’s Hospital showed a decreasing trend year by year,while the positive rate of patients aged 41-60 years old showed a rising trend year by year.The HCV epidemic genotypes in Sichuan area showed the distribution characteristics of multiple genotypes,mainly cases were type 1b.Understanding the basic situation of HCV-infected patients and common classification is of great value for clinical detection and treatment.更多还原