【作者】 王浩然；

【导师】 于大海；

【作者基本信息】 吉林大学 ， 生物工程（专业学位）， 2020， 硕士

【摘要】 益生菌,是一类主要定植在宿主的肠道、皮肤等部位,对宿主的相关系统的微生态稳定起到重要调节作用的活性微生物的总称。随着人们对益生菌研究的逐步深入,如何进一步发挥益生菌调理肠道的作用逐渐成为热点话题。目前已有很多的报道表明,传统中药,尤其是部分药食同源类和补益类中药能够有效的促进人体内的益生菌生长,并能抑制体内有害菌的繁殖。与此同时,中药进入人体并被分解后,还能起到直接拮抗病原菌和增强人体抵抗力等作用。综上所述,如果将部分中药与益生菌产品联合使用,协同起效,不仅可以发挥中药治疗特性,还可以进一步显示益生菌调理肠道功能的优越性。本论文研究了中药对于益生菌体外生长的影响,并对中药-益生菌复合微生态制剂的成分进行了配方设计与优化,具体的实验内容及结果如下:中药对益生菌生长的影响。本实验研究了多种传统中药(包括补益药、理血药等等)中对瑞士乳杆菌、干酪乳杆菌和副干酪乳杆菌体外生长的影响。我们的研究表明,本实验所选择的部分补益类的中药能够显著促进瑞士乳杆菌和副干酪乳杆菌的体外生长,且通过流式细胞实验证实,它们不会影响益生菌的活菌比例,只有远志和绿茶表现出对瑞士乳杆菌和副干酪乳杆菌的轻微抑制;适宜浓度的巴戟天、韭菜籽等中药对益生菌的促生长作用较为显著。对于干酪乳杆菌,少数补益类中药能够显著影响益生菌的生长,其余大部分中药对益生菌的生长基本没有影响。这些结果与之前的研究结论相吻合。部分中药与益生菌的联合使用,不仅对益生菌的生长有积极影响,还可以利用益生菌对中药进行生物转化,增加中药的利用度。为了最大化的利用中药与益生菌间的协同作用,为中药与益生菌联合使用在实际生产中的应用奠基,我们对中药-益生菌复合微生态制剂进行了设计和初步优化。我们依据上一步中筛选得到的较好的中药-益生菌组合,设计了中药-益生菌复合微生态制剂配方,并针对影响菌体生长的10个因素(所选用中药及蛋白胨、葡萄糖、酵母粉等培养基成分)使用响应面法进行了优化,验证实验结果表明,经优化后,制剂中活菌数较之正常培养的益生菌提高约3.2倍,可以进一步发挥了中药-益生菌联合在调理肠道功能上的作用。利用中药和益生菌的相互作用,能够为益生菌保健领域的进一步发展提供帮助。本实验探究了不同种类的中药对益生菌生长的影响。证实了部分中药如韭菜籽、巴戟天等确实可以促进益生菌的生长,且不影响益生菌活性。对中药-益生菌复合微生态制剂进行优化设计后,活菌数获得提高。目前对于益生菌与中药互作的研究尚不多,我们希望通过我们的研究可以为中药与益生菌的联合使用奠定理论基础,为益生菌-中药的联用进一步应用在生产和生活中进行奠基。更多还原

【Abstract】 Probiotics are a group of active microorganisms that are mainly colonized in the intestinal tract,skin,and other parts of the host and play an important role in regulating the micro-ecological balance of the host’s related systems.With the gradual deepening of people’s research on probiotics,how to further play the role of probiotics in regulating the intestinal tract on the premise of maintaining or increasing the number of probiotics is becoming a hot topic.At present,there have been many reports that traditional Chinese medicine,especially some medicine and food homology and supplementary Chinese medicine,can effectively promote the growth of probiotics in the human body and can inhibit the reproduction of harmful bacteria in the body.At the same time,after the Chinese medicine enters the body and is broken down,it can also directly antagonize the pathogenic bacteria and enhance the body’s resistance.In summary,if some Chinese medicines are used in combination with probiotic products to synergize,they can exert the therapeutic properties of traditional Chinese medicines.This thesis studies the influence of traditional Chinese medicine on the growth of probiotics in vitro,and optimizes the design of the composition of traditional Chinese medicine-probiotic composite micro-ecological preparation.Then the specific experimental content and results are as follows:The effect of Chinese medicine on the growth of probiotics.In this experiment,the effects of various traditional Chinese medicines(including tonic medicines,blood remedies,etc.)on the growth of Lactobacillus helveticus,Lactobacillus casei and Lactobacillus paracasei in vitro were studied.Our research shows that some of the supplementary Chinese medicines selected in this experiment can significantly promote the in vitro growth of Lactobacillus helveticus and Lactobacillus paracasei,and it has been confirmed by flow cytometry that they will not affect the proportion of probiotics.Polygala and green tea showed a slight inhibition of Lactobacillus helveticus and Lactobacillus paracasei;Chinese medicines such as Morinda officinalis and leek seeds had a significant growth promoting effect on probiotics.For Lactobacillus casei,a few supplementary Chinese medicines can significantly affect the growth of probiotics,and most of the remaining Chinese medicines have basically no effect on the growth of probiotics.These results are consistent with previous research conclusions.The joint use of some Chinese medicines and probiotics not only has a positive effect on the growth of probiotics,but also can use probiotics to biotransform Chinese medicines and increase the utilization of Chinese medicines.In order to maximize the synergy between Chinese medicine and probiotics,and lay the foundation for the application of Chinese medicine and probiotics in actual production,we designed and preliminary optimized the Chinese medicine-probiotic composite micro-ecological preparation.Based on the better Chinese medicine-probiotic combination screened in the previous step,we designed the Chinese medicine-probiotic compound microecological preparation formula,and aimed at 10 factors that affect the growth of the bacteria(selected Chinese medicine and peptone,glucose,yeast powder)And other medium components)were optimized using response surface method.The results of verification experiments showed that after optimization,the number of viable bacteria increased by about 3.2 times compared with the normal cultured probiotics,which can further exert the combination of traditional Chinese medicine and probiotics in regulating intestinal Functional role.Using the interaction between Chinese medicine and probiotics can help further development of probiotic health care.In this experiment,we explored the effects of different kinds of traditional Chinese medicine on the growth and activity of probiotics.It is confirmed that some Chinese herbal medicines can indeed promote the growth of probiotics,and do not affect the activity of probiotics.After the optimized design of the traditional Chinese medicine-probiotic compound micro-ecological preparation,the number of viable bacteria was improved.At present,there is not much research on the interaction between probiotics and Chinese medicine.We hope that through our research we can lay a theoretical foundation for the joint use of Chinese medicine and probiotics,and lay the foundation for the further application of the combination of probiotics and Chinese medicine in production and life.更多还原