【作者】 李伟；

【导师】 殷宪敏；

【作者基本信息】 泰山医学院 ， 儿科学（专业学位）， 2016， 硕士

【摘要】 目的通过查找、归纳、总结文献了解儿童急性下呼吸道感染(ALRTI)常见病毒生物学、临床特征及流行病学。通过收集下呼吸道感染患儿的咽拭子,进行病毒检测,用来对泰安地区儿童下呼吸道感染病毒病原学进行研究,为儿童急性呼吸道感染的预防和诊疗提供依据。研究方法以2014年2月~2015年2月,在泰山医学院附属医院儿科就诊及住院治疗的临床诊断为ALRTI的患儿共202例,就诊当日或次日采集患儿咽拭子一份,采用适时荧光逆转录聚合酶联反应方法来进行咽拭子病毒检测,“常见呼吸道病毒,包括呼吸道合胞病毒(RSV)、鼻病毒(RV)、副流感病毒(PIV)1-3型、甲型及乙型流感病毒(IFA、IFB)、腺病毒(ADV)、肠道病毒(EV)、冠状病毒(HCoV)、人偏肺病毒(hMPV)及人博卡病毒(HBoV)。”~i结果202份检测标木中,检测出呼吸道病毒95株,检出率为47.02%。RSV病毒45株,其中RSVA有36株,RSVB有9株;副流感病毒35株,其中有4株1型,29株2型,2株3型;RhinoV病毒4株;腺病毒(ADV)6株;肠道病毒(HEV)10株;流感病毒2株,均为甲型流感病毒;冠状病毒3株,其中有2株OC43型、有1株NL63型,未检测到冠状病毒229E、HKU1型;偏肺病毒及博卡病毒各检测到3株。病毒构成差异有统计学意义(P>0.05)。阳性标本中合并感染2种病毒共17份,占总感染数的37.78%,混合感染最多的是呼吸道合胞病毒及副流感病毒。呼吸道病毒检出阳性率冬春季较高。病毒阳性率随着年龄的增长呈现下降的趋势。结论泰安地区儿童急性下呼吸道感染以呼吸道合细胞病毒,副流感病毒为主,好发于冬春季节,随着患儿年龄的增加病毒分离率呈现下降趋势。更多还原

【Abstract】 ObjectiveBy searching,inducting,summarizing the literature to understand children with acute lower respiratory tract infections(ALRTI)common virus biology,clinical features and epidemiology.To survey the virus etiology in nasopharyn-geal aspirate(NPA)collected from children with acute lower respiratory tract infections(ALRTI)in Taian.MethodTwo hundred and two NPA samples were collected to detect 10 respiratory virus by multiplex PCR,including respiratory syncytial viruses(RSV)A and B,rhinovirus(RhinoV),parainfluenza virus(PIV)1,2,3,flu virus(Flu)A and B,adenovirus(ADV),humanmetapneumovirus(HMPV),coronavirus(CoV)OC43/HKU1 and CoV 229E/NL63,Human metapneumovirus(HMPV),Human Bocavirus(HBoV),were detected by PCR.RosultThe positive pathogenic respiratory viruses was 95(47.02%)in 202 NPA samples.Of95 virus positive patients,45 were RSV infection,RSVA infection were 36,RSVB infection were 9;35 were PIV infection,PIV type 1 infection were 4 cases,PIV type 2 infection were29 cases,PIV type 3 infection were 2 cases;Including 4 cases of Rhinovirus,6 of Adenovirus infection,10 of enterovirusinfection,2 of influenza virus A infection,3 of human coronavirus infection,two cases wereHCoV-OC43,one case was HCoV-NL63.Of 95 virus positive patients,17(37.78%)were 2 viruses co-infection.The most frequently detected virus was RSV,followed by PIV.Respiratory virus positive rate higher in winter and spring.The positive rate of respiratory virus with age showed a downward trend.ConclusionRSV and PIV was the predominant pathogen in children with ALRTI in Taian area.Respiratory virus positive rate higher in winter and spring.The positive rate of respiratory virus with age showed a downward trend.更多还原