【作者】 张明；

【导师】 周成；

【作者基本信息】 昆明理工大学 ， 环境工程， 2019， 硕士

【摘要】 难降解有机废水难以被有效且低成本处理,但白腐真菌因能降解多种难降解有机污染物而在此类废水治理中有巨大的应用潜力。然而,白腐真菌反应器难以实现长期的连续运行。课题组前期基于脉管载体和蒸笼反应器构建出好氧丝状生物膜,首次实现了在开放体系下对于垃圾渗滤液和焦化废水的长期连续处理,但尚未考察白腐真菌好氧丝状生物膜在上述废水中的连续生长机制。针对此问题,本论文考察了P.chrysosporium好氧丝状生物膜的形成和在反应器内的连续生长过程,结果表明:·在挂膜阶段,P.chrysosporium孢子能够附着到脉管载体的细纤维丝中,继而萌发生长,最终形成覆盖于载体表面的好氧丝状生物膜;·在P.chrysosporium好氧丝状生物膜投加到蒸笼反应器中,生物膜着色,表明其首先对废水中的污染物质进行吸附;另外,其连续合成木质素过氧化物酶(Lip)降解废水中的COD和氨氮。这表明:P.chrysosporium好氧丝状生物膜进行了吸附-降解-生长的过程;·在垃圾渗滤液和焦化废水处理中,P.chrysosporium好氧丝状生物膜的外膜会被撕裂并且成片脱落,但固定于载体细纤维丝中的内膜继续存在于载体细纤维丝中,继续生长,再次形成P.chrysosporium好氧丝状生物膜的新外膜,增加P.chrysosporium好氧丝状生物膜厚度。随后,新形成的外膜又会出现撕裂和脱落。基于此,论文提出基于脉管载体构建的P.chrysosporium好氧丝状生物膜能够在难降解有机废水中连续生长的机制:P.chrysosporium好氧丝状生物膜的外膜脱落-内膜向外生长形成新外膜-新外膜再脱落-内膜再次生长的循环生长过程,即脱落-外推式生长的循环生长机制。连续生长的实现可归因于:(1)脉管载体,其上的好氧丝状生物膜可以从载体内和载体外双向向膜供应氧气和营养物质,实现膜的连续生长;(2)蒸笼反应器,其可实现P.chrysosporium好氧丝状生物膜的分层和静止培养,减少对膜的破坏;(3)序批式的运行方式,其可以减少其它微生物和代谢物等对膜的干扰。更多还原

【Abstract】 It is a challenge for the effective,low-cost treatment of refractory organic wastewater.The white-rot fungi have great potential to treat it due to their unique ability to degrade refractory organic pollutants.However,white-rot fungal treatment system has been impeded owing to short-term fungal activities in reactor.For the first time,our research group has successfully achieved the long-term continuous growth of P chrysosporium aerobic filament biofilm in landfill leachate and coking wastewater under non-sterile conditions,which based on vascular carrier s and Zheng-Long reactors.But the mechanism involved the long-term continuous growth of P chrysosporium aerobic filament biofilm in wastewater remains to be studied.In order to solve this problem,the formation and growth process of P chrysosporium aerobic filamentous biofilm in wastewaterwas were investigated.The experimental results showed that:· In biofilm formation stage,the spores of P.chrysosporiumcan attached to vascular carrier,germinated in it to form P.chrysosporiumaerobic filamentous biofilm and then grew to cover the surface of vasc ular carrier.· When P.chrysosporium aerobic filamentous biofilms were added to wastewater,they first adsorbed pollutants and changed their colour.In addition,they maintained the continuous Li P production to remov e COD and ammonium nitrogen,which suggested that P.chrysosporium aerobic filamentous biofilm undergoe d adsorption,degradation and growth process.· In landfill leachate and coking wastewater,the outer layers of P.chrysosporium aerobic filamentous biofilms were torn open and gradually exfoliated,but their inner layer still fixed in vascular carrier and grew to increase the thickness of P.chrysosporium aerobic filamentous biofilm,and developed new outer layers of P.chrysosporium aerobic filamentous biofilm s.The new outer layers were torn open and gradually exfoliated.Based on those results,it proposed that the growth mechanism of P.chrysosporium aerobic filamentous biofilm in refractory organic wastewater was the cyclic growth process involving the exfoliation of outer layer-continuous growth of inner layer-the exfoliation of new outer layer.The continuous growth of P chrysosporium aerobic filament biofilm was attributable to(1)a vascular carrier designed to form aerobic filamentous fungal biofilm in/on its surface and to achieve the continuous supply of nutrient and oxygen for its inner layer to keep continuous growth of inner layer,(2)a Zheng-Long rector designed to achieve the layered,static culture of aerobic filamentous biofilm and to decrease some damages for biofilm,and(3)An appropriate reactor operation strategy(Sequencing Batch)used to decrease the influence of metabolites and bacteria on aerobic filamentous biofilm.更多还原