【作者】 王蕾；

【导师】 刘杰；

【作者基本信息】 青岛大学 ， 口腔临床医学， 2019， 硕士

【摘要】 目的:钛是口腔种植领域一种良好的种植体材料,但易发生生物老化从而减慢成骨细胞对种植体生物学反应。本实验研究的目的是通过低温氩氧等离子体(LTAOP)即时活化已经发生生物老化的钛表面,来增强钛对成骨细胞的生物相容性,从而加快种植体骨结合的进程。方法:以氩气+氧气的混合气体作为输入气体产生低温氩氧等离子体对已经发生生物老化的喷砂酸蚀钛表面(SLA Ti)和光滑钛表面(SM Ti)进行即时的活化处理,将经过低温氩氧等离子体处理的两种钛材料表面作为实验组,即SM Ti+LTAOP和SLA Ti+LTAOP。将未经过低温氩氧等离子体处理的两种钛材料表面作为对照组,即SM Ti和SLA Ti。同时将体外培养的Sprague Dawley(SD)大鼠的成骨细胞分别培养在LTAOP处理前后的SM Ti和SLA Ti表面上。通过扫描电镜来观察LTAOP处理前后成骨细胞的黏附情况,利用激光共聚焦显微镜观察经过LTAOP处理前后的成骨细胞的细胞骨架肌动蛋白(Actin)的伸展情况,通过MTT来检测经过LTAOP处理前后各组细胞在1,3,5天的增殖情况,通过检测经LTAOP处理前后的骨钙素(OC)在第7天和第14天的分泌量来评估成骨细胞的分化情况。结果:1.成骨细胞的黏附情况:通过扫描电镜观察,实验组的成骨细胞在材料表面的黏附面积更大,且差别具有统计学意义(P<0.05)。2.成骨细胞MTT的检测情况:经过LTAOP处理的实验组更有利于成骨细胞的增殖,且差别具有统计学意义(P<0.05)。3.细胞骨架肌动蛋白的伸展情况:实验组成骨细胞的细胞骨架肌动蛋白铺展的更加充分且相互交联形成网状,铺展面积更大且差别具有统计学意义(P<0.05)。4.成骨细胞骨钙素(OC)的分泌情况:实验组在第7天和第14天的骨钙素分泌量明显高于对照组,差别具有统计学意义(P<0.05)。结论:研究表明,经过低温氩氧等离子体活化的钛表面可增强成骨细胞的黏附、增殖和分化,加速成骨细胞与钛表面的早期骨整合。本实验为种植体在植入前即时活化种植体表面提供了一种新的思路。更多还原

【Abstract】 Objective: Titanium is a good implant material in the field of oral implants,but it is prone to biological aging and slows the biological response of osteoblasts to implants.The purpose of this experimental study was to enhance the biocompatibility of titanium to osteoblasts by activating low-temperature argon-oxygen plasma(LTAOP)to instantly activate the biologically aged titanium surface,thereby accelerating the process of implant osseointegration.Methods: a mixed gas of argon + oxygen is used as an input gas to generate a low-temperature argon-oxygen plasma to immediately perform the bio-aging of the blasted titanium surface(SLA Ti)and the smooth titanium surface(SM Ti).The activation treatment,the surface of two titanium materials treated by low temperature argon oxygen plasma was used as the experimental group,namely SM Ti+LTAOP and SLA Ti+LTAOP.The surfaces of two titanium materials which were not subjected to low temperature argon-oxygen plasma treatment were used as a control group,namely SM Ti and SLA Ti.At the same time,osteoblasts of Sprague Dawley(SD)rats cultured in vitro were cultured on the surface of SM Ti and SLA Ti before and after LTAOP treatment.The adhesion of osteoblasts before and after LTAOP treatment was observed by scanning electron microscopy.The cytoskeletal actin(Actin)of osteoblasts before and after LTAOP treatment was observed by laser confocal microscopy.The results were detected by MTT before and after LTAOP treatment.The proliferation of each group of cells was evaluated at 1,3,and 5 days,and the differentiation of osteoblasts was evaluated by detecting the amount of osteocalcin(OC)secreted on days 7 and 14 before and after LTAOP treatment.Results: 1.Adhesion of osteoblasts: the scanning area of osteoblasts on the surface of the experimental group was larger than that of the control group by scanning electron microscopy,and the difference was statistically significant(P<0.05).2.Detection of osteoblast MTT: the experimental group treated with LTAOP was more favorable for the proliferation of osteoblasts,and the difference was statistically significant(P<0.05).3.Stretching of cytoskeletal actin: compared with the control group,the cytoskeletal actin of the experimental bone cells spread more fully and cross-linked to form a network,and the spread area was larger and the difference was statistically significant(P<0.05).Osteoblast osteocalcin(OC)secretion: The osteocalcin secretion of the experimental group on day 7 and day 14 was significantly higher than the control group,the difference was statistically significant(P <0.05).Results: 1.Adhesion of osteoblasts: The scanning area of osteoblasts on the surface of the experimental group was larger by scanning electron microscopy,and the difference was statistically significant(P<0.05).2.Detection of osteoblast MTT: The experimental group treated by LTAOP was more beneficial to the proliferation of osteoblasts,and the difference was statistically significant(P<0.05).3.Stretching of cytoskeletal actin: The cytoskeletal actin of the experimental bone cells was spread more fully and cross-linked into a network,and the spreading area was larger and the difference was statistically significant(P<0.05).Osteoblast osteocalcin(OC)secretion: The osteocalcin secretion of the experimental group on day 7 and day 14 was significantly higher than the control group,the difference was statistically significant(P <0.05).Conclusion: Studies have shown that titanium surface activated by low temperature argon-oxygen plasma can enhance the adhesion,proliferation and differentiation of osteoblasts and accelerate the early osseointegration of osteoblasts and titanium surface.This experiment provides a new idea for implants to activate the implant surface immediately before implantation.更多还原