【作者】 张丹；

【导师】 贾新华；

【作者基本信息】 山东中医药大学 ， 中医内科学（专业学位）， 2018， 硕士

【摘要】 目的:本研究通过文献检索,整理、归纳中医药治疗间质性肺病的临床应用规律,并观察清热解毒类中药对博来霉素致肺纤维化大鼠的治疗作用,尝试归类前人的临证思想,同时在这个基础上,探究清热解毒类中药与抗纤维化之间的各种机制。方法:1.临床应用规律的研究:以“知网”为目标检索数据库,检索有关中药对间质性肺病的疗效的相关文献,并基于频数统计的研究方法,将使用中药对肺纤维化病症治疗的频数与中药的类型进行统计。2.动物实验研究:将大鼠168只随机分成7组,以气管滴入法搭建应用博来霉素诱导肺组织纤维化的大鼠模型,并使用对应药物进行干预,观察大鼠的一般情况,分别取肺组织等标本进行检测,采用SPSS17.0统计软件处理相关实验数据。结果:1.治疗肺纤维化所用药物所使用的中药种类总数为246,使用的总频数为1613。而其中,频数大于10的共计37种,主要有甘草、当归、黄芪以及川芎等。2.治疗肺纤维化所用药物种类所使用的中药共计18种,而其中药物使用占比频数大于10%的中药大类有:补虚药、活血化瘀药、化痰止咳平喘药、清热药。3.清热类药物中应用频次最高的是清热解毒药,其中鱼腥草、金银花、连翘应用频次较高。4.清热解毒类中药治疗组明显改善肺纤维化模型大鼠肺组织的病理学改变。结论:清热解毒类中药能够延缓肺纤维化进程,其作用机制可能与减轻炎症反应、抗氧化损伤、影响BMP通路等多种途径有关。更多还原

【Abstract】 Objective: Trough retrieving relevant literature,this paper collated clinical application of clinical application of TCM in the treatment of interstitial lung disease and observed the therapeutic effect of antipyretic and detoxifying TCM on bleomycin-induced pulmonary fibrosis in rats to sum up the previous clinical thinking,and on this basis to multiple mechanisms of anti-fibrosis of heat-clearinging and detoxifying TCM.Method: 1.Study on clinical application laws: This paper retrieved the literature on TCM treatment of the interstitial lung disease in CNKI,and then sorted out the frequency of dug and the classification of the TMC of the treatment of the TMC in pulmonary fibrosis.2.Animal experimental study: 168 rats were randomly divided into 7 groups;established the rat modelbleomycin-induced pulmonary fibrosis and gave the corresponding drug intervention,then observed the general condition of the rats;teated the lung issue and other criteria respectively,and used SPSS17.0 statistical software to deal with relevant experimental data.Results: 1.The drugs used in the treatment of pulmonary fibrosis involve a total of 246 and frequency for 1613,among which the frequency of 37 kinds of drugs,including radix astragalus root and rhizome,and so on.2.The types of drugs used in the treatment of pulmonary fibrosis involved 18 kinds of TCM,among which these drugs whose drug frequency constitutive proportion more than 10% are tonic drugs,blood-activating and stasis-removing drugs,Huatanzhike asthma and antipyretics 3.The highest frequency of use of the 3.antipyretic drugs is the antipyretic and detoxifying drugs,of which Houttuynia,honeysuckle and forsythia are more frequently used.4.Heat-clearinging and detoxifying TCM can reduce the degree of pulmonary fibrosis,in which forsythia can delay the process of pulmonary fibrosis by influencing the expression of SOD,MDA,BMP-4.Conclusion: Heat-clearinging and detoxifying TCM can delay the process of pulmonary fibrosis,and its mechanism may be related to many ways,such as reducing inflammatory reaction,anti oxidative injury and affecting the BMP pathway.更多还原