【作者】 张斌；

【导师】 郭辉； 钱俊青； 陈春；

【作者基本信息】 浙江工业大学 ， 药学， 2014， 硕士

【摘要】 橘皮柠檬烯具有抗炎、抗肿瘤、祛痰止咳等药用功效,且安全性好,深层次开发利用柠檬烯,具有极为重要的意义。本论文首先对柑橘精油进行纯化研究,获得柠檬烯分离工艺。以柑橘粗油作为原料,通过控制温度和压力进行减压蒸馏,以d-柠檬烯含量为指标,得到较好的工艺条件。结果表明温度越高,真空度越高,分离效果越好。温度为90℃,压力为30 hPa时为分离纯化工艺较好的条件。得到的柠檬烯对光照、酸和高温均很稳定。其次,采用机械搅拌结合超声的方法,以分离纯化得到的柠檬烯为原料制备柠檬烯乳剂,以乳剂平均粒径的大小为指标,得到最佳单因素为乳化剂HLB=12,水浴温度=60℃,油相的用量=16%,油相比乳化剂比例S0 ratio=0.6,搅拌时间=15min,超声功率=750w,超声时间=8min,在此实验结果的基础上,选取三个影响较为显著的因素:油相比例,油相比乳化剂比例(S0 ratio)和搅拌时间进行响应面优化实验。响应面优化实验结果表明,油相比例,油相比乳化剂比例(S0 ratio)和搅拌时间对柠檬烯乳剂粒径大小的影响不是简单的线性关系。分析得到最小响应值(R1)时对应的柠檬烯乳剂粒径的最佳条件为:油相比例17.94,油相比乳化剂比例0.686,搅拌时间6.25min,理论最佳粒径为11.39。考虑到实际操作和生产的便利,将最佳条件定为:油相比例18.0%,油相比乳化剂比例0.69,搅拌时间6min,并进行验证试验,得到的乳剂平均粒径为11.49,基本与预测值相符合。通过测量乳剂的粒径大小,离心稳定常数,zeta电位,pH,对乳剂的性状及稳定性进行表征。结果乳剂平均粒径为11.49,离心稳定常数为 13.82%,zeta 电位为-25.97,pH 为 7.22。最后,采用二甲苯致小鼠耳肿胀试验及醋酸致小鼠毛细血管通透性影响试验进行柠檬烯乳剂和软膏剂的皮肤抗炎研究。结果表明,此柠檬烯乳剂具有显著的皮肤抗炎效果;二甲苯致小鼠耳肿胀试验表明,柠檬烯软膏剂具有较好的皮肤抗炎效果。两者相比较,乳剂的效果明显好于软膏剂。更多还原

【Abstract】 Limonene from Citrus Peel have more advantages like wide source,multi-function,safety,xploit deeply and comprehensively utilize of limonene to renew Citrus Peel resources,is very important for economic and social significance.First,explore the better purification process and its stability of Citrus Peel essential oils of limonene.With coarse extraction of Citrus Peel essential oil as raw material,Vacuum distilling by controlling the temperature and vacuum degree,limonene content as the index,to get the best technological conditions.The results showed that the higher of the temperature and the vacuum degree,the separation effect activity will be better.The better condition for purification process was 90℃ and 30 hPa.The limonene was very stable under the conditions of light,acid and high temperature.Secondly,using the method of mechanical agitation combined with ultrasonic to make limonene emulsion,with the average particle size of emulsion as the index,and do the single factor experiment,got the best single factor was emulsifier:HLB=12,water bath temperature=60℃,the dosage of the oil phase=16%,oil/emulsifier(S0 ratio)=0.6,stirring time=15 min,ultrasonic power=750 w,ultrasonic time=8 min,based on these results of single factor experiments,selecting three significant difference factors:oil phase proportion,oil/emulsifier(S0 ratio)and mixing time,to do the response surface optimization experiment.The results of response surface optimization showed that the effect of limonene emulsion particle size from proportion of oil phase,oil/emulsifier(S0 ratio)and the mixing time was not a simple linear relationship.the best condition to get limonene of emulsion particle size corresponding with minimum response values,as follows:the oil phase ratio=17.94,oil/emulsifier=0.686,the mixing time=6.25 min,the theory of optimum particle size was 11.39.Considering the convenience of practical operation and production,the best conditions was:oil phase ratio=18.0%,oil/emulsifier ratio=0.69,the mixing time=6 min,get the best particle size was 11.49.According to the optimal conditions for preparation citrus essential oil emulsion,by measuring the particle size of emulsion,the centrifugal stability constant,zeta potential,pH,to represe the properties and stability of the emulsion.And we got the results that the average particle size is 11.49,emulsion centrifugal stability constant is 13.82%,zeta potential is-25.97,pH is 7.22.On the other hand,we compared anti-inflammatory activity of limonene ointments with emulsion.Finally,we researched xylene induced mouse ear swelling test and influencing test of blood capillary permeability of the acetic acid in mice of limonene emulsion.And the results showed that this limonene emulsion had significant anti-inflammatory effect for skin;Xylene induced mouse ear swelling test showed that limonene soft paste has better anti-inflammatory effect.In other words,the effect of the emulsion was much better than the ointments for anti-inflammatory.更多还原